Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1792Y] to pan Cadherin - Intercellular Junction Marker
  • Suitable for: WB, IP, Flow Cyt, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker
    See all pan Cadherin primary antibodies

  • Description

    Rabbit monoclonal [EPR1792Y] to pan Cadherin - Intercellular Junction Marker

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Mouse
    Rat
    Human
    IP
    Rat
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human pan Cadherin (C terminal). The exact sequence is proprietary.
    Database link: P19022

  • Positive control

    • WB: C6 whole cell lysate. Mouse heart tissue lysate. Human, mouse, and rat brain tissue lysates; ICC: MCF7 cells; IHC-P: Rat, mouse and human cardiac muscle tissue; human and rat colon and mouse testis tissue; IP: Rat heart lysate; Flow Cyt: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
    Western blot - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
    All lanes : Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034) at 1/50000 dilution (purified)

    Lane 1 : C6 (Rat glial tumor glial cell) whole cell lysate
    Lane 2 : Human brain lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 100 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cardiac muscle tissue sections labeling pan Cadherin with purified ab51034 at 1/500 dilution (0.26 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
    Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

    Immunocytochemistry/Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cells labeling pan Cadherin with purified ab51034 at 1/250. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. 

    ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 was used as counterstain antibody.

    Confocal image showing membranous staining in MCF7 cells.

  • Flow Cytometry - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
    Flow Cytometry - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling pan Cadherin with purified ab51034 at 1/20 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunoprecipitation - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
    Immunoprecipitation - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

    Purified ab51034 at 1/20 dilution (0.6 µg) immunoprecipitating pan Cadherin in Rat heart lysate.
    Lane 1 (input): Rat heart lysate 10 µg
    Lane 2 (+): ab51034 + Rat heart lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51034 in Rat heart lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 140 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue sections labeling pan Cadherin with purified ab51034 at 1/500 dilution (0.26 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue sections labeling pan Cadherin with purified ab51034 at 1/500 dilution (0.26 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

    Immunohistochemical analysis of Paraffin-embedded human colon tissue sections labeling pan Cadherin with unpurified ab51034 at 1/100. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).

    Positive staining on human colon.

  • Western blot - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
    Western blot - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
    Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034) at 1/50000 dilution (purified) + Mouse heart lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 100 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

    Immunohistochemical analysis of Paraffin-embedded rat colon tissue sections labeling pan Cadherin with unpurified ab51034 at 1/100. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).

    Positive staining on rat colon.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

    Immunohistochemical analysis of Paraffin-embedded mouse testis tissue sections labeling pan Cadherin with unpurified ab51034 at 1/100. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).

    Positive staining on mouse testis.

  • OI-RD Scanning - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
    OI-RD Scanning - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
    Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

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