HRP Anti-pan Cadherin antibody [mAbcam22744] (ab197725)
![HRP Anti-pan Cadherin antibody [mAbcam22744] (ab197725)](https://www.abcam.com/ps/products/197/ab197725/Images/ab197725-238353-anti-pan-cadherin-antibody-mabcam22744-hrp-western-blot.jpg "HRP Anti-pan Cadherin antibody [mAbcam22744] (ab197725)")
Key features and details
- HRP Mouse monoclonal [mAbcam22744] to pan Cadherin
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
- Isotype: IgG1
Overview
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Product name
HRP Anti-pan Cadherin antibody [mAbcam22744]
See all pan Cadherin primary antibodies -
Description
HRP Mouse monoclonal [mAbcam22744] to pan Cadherin -
Host species
Mouse -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Rat, mouse and human heart tissue lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
mAbcam22744 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - HRP Anti-pan Cadherin antibody [mAbcam22744] (ab197725)All lanes : HRP Anti-pan Cadherin antibody [mAbcam22744] (ab197725) at 1/5000 dilution
Lane 1 : Heart (Rat) Tissue Lysate
Lane 2 : Heart (Mouse) Tissue Lysate
Lane 3 : Heart (Human) Tissue Lysate - adult normal tissue
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 125 kDa
Observed band size: 125 kDa
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab197725 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
