FITC Anti-ZO1 tight junction protein antibody [mAbcam 61357] (ab150266)
![FITC Anti-ZO1 tight junction protein antibody [mAbcam 61357] (ab150266)](https://www.abcam.com/ps/products/150/ab150266/Images/ab150266-245645-anti-zo1-tight-junction-protein-antibody-mabcam-61357-fitc-flow-cytometry.jpg "FITC Anti-ZO1 tight junction protein antibody [mAbcam 61357] (ab150266)")
Key features and details
- FITC Mouse monoclonal [mAbcam 61357] to ZO1 tight junction protein
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Overview
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Product name
FITC Anti-ZO1 tight junction protein antibody [mAbcam 61357]
See all ZO1 tight junction protein primary antibodies -
Description
FITC Mouse monoclonal [mAbcam 61357] to ZO1 tight junction protein -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Synthetic peptide corresponding to ZO1 tight junction protein aa 750-850 conjugated to keyhole limpet haemocyanin.
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Positive control
- Flow Cyt: MCF7 cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
mAbcam 61357 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Research areas
Images
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Flow Cytometry - FITC Anti-ZO1 tight junction protein antibody [mAbcam 61357] (ab150266)
Overlay histogram showing MCF7 cells stained with ab150266 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab150266, 0.1μg/1x106 cells) for 30 min at 22ºC.
Isotype control antibody (black line) was mouse IgG1 [ICIG1] FITC (ab91356) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
