Anti-ZO1 tight junction protein antibody (ab96587)
Key features and details
- Rabbit polyclonal to ZO1 tight junction protein
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ZO1 tight junction protein antibody
See all ZO1 tight junction protein primary antibodies -
Description
Rabbit polyclonal to ZO1 tight junction protein -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P MouseHumanWB MouseHuman
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Immunogen
Recombinant protein fragment corresponding to a region within amino acids 1 - 266 of Human ZO1 tight junction protein.
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Positive control
- WB: HEK-293T, HeLa, Neuro-2a, C8-D30, NIH/3T3 and C2C12 cell lysate, mouse testis tissue lysate; IHC-P: Mouse testis and intestine tissues; H661 xenograft tissue. IF: NTERA-2 cl.D1 [NT2/D1] cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody (ab96587) Image from Hu X et al., Reprod Biol Endocrinol., 16, 91, Fig 6.; doi:10.1186/s12958-018-0401-7
Hu X, Ding Z, Hong Z, et al. used ab96587 in immunofluorescence analysis.
Immunofluorescence staining MVH and ZO-1 in human testis specimens. Response group 1, Response group 2A and Response group 2B during the secondary TESE.
Scale bar, 100 μm.
Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.
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Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody (ab96587) Image from Jun-Chao Lin et al., Cell Prolif., 52, e12547, Fig 3.; doi: 10.1111/cpr.12547.Lin JC, Wu JQ, Wang F, et al. used ab96587 in immunofluorescence analysis.
QBD preserves intestinal barrier function in DSS‐treated mice. Paraffin‐embedded colon tissues were used to determine zonula occludens‐1 (ZO‐1), followed by FITC‐labelled secondary antibody and observed by fluorescence microscopy (red staining).
Nuclei were stained with 4,6‐diamidino‐2‐phenylindole (DAPI; blue staining). First panel: treatment with water; Second panel: treatment with mesalazine; Third panel: treatment with Dextran sulphate sodium; Fourth panel: treatment with QingBai decoction.
Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.
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Western blot - Anti-ZO1 tight junction protein antibody (ab96587)All lanes : Anti-ZO1 tight junction protein antibody (ab96587) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 187 kDa5% gel.
Running condition: 80V, 15min; 140V, 40min.
Transfer condition:Semi-dry, 18 V, 60min (Nitrocellulose membrane)
Blocking condition: 5% non-fat milk in TBST, RT, 60min.
Primary antibody incubation: 4°C overnight.
Secondary antibody incubation: Room temperature for 1 hour.
Washing condition: 5 ml TBST, 4 x 5min.
ECL detection.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody (ab96587)Immunohistochemical analysis of paraffin-embedded mouse intestine tissue staining ZO1 tight junction protein at cell membrane and cytoplasm with ab96587 at 1/500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody (ab96587)Immunofluorescence analysis of ice-cold methanol-fixed (5 min) NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) cells labeling ZO1 tight junction protein at cell membrane (green) with ab96587 at 1/200 dilution. Blue: Hoechst 33342 staining.
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Western blot - Anti-ZO1 tight junction protein antibody (ab96587)All lanes : Anti-ZO1 tight junction protein antibody (ab96587) at 1/500 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
Lane 2 : C8-D30 (mouse astrocyte type III cerebellum cell line) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 187 kDa5% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody (ab96587)Immunohistochemical analysis of paraffin-embedded mouse testis staining ZO1 tight junction protein at cell membrane and cytoplasm with ab96587 at 1/500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody (ab96587)Immunohistochemical analysis paraffin-embedded H661 xenograft tissue stained for ZO1 tight junction protein using ab96587 at 1/250 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Western blot - Anti-ZO1 tight junction protein antibody (ab96587)All lanes : Anti-ZO1 tight junction protein antibody (ab96587) at 1/1000 dilution
Lane 1 : Non-transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : ZO1 tight junction protein shRNA-transfected HeLa whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 187 kDa5% SDS-PAGE
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Western blot - Anti-ZO1 tight junction protein antibody (ab96587)Anti-ZO1 tight junction protein antibody (ab96587) at 1/500 dilution + Mouse testis tissue lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 187 kDa5% SDS-PAGE
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Western blot - Anti-ZO1 tight junction protein antibody (ab96587)Anti-ZO1 tight junction protein antibody (ab96587) at 1/500 dilution + C2C12 (mouse myoblast cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 187 kDa5% SDS-PAGE
