Anti-pan Cadherin antibody (ab6529)
Key features and details
- Rabbit polyclonal to pan Cadherin
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-pan Cadherin antibody
See all pan Cadherin primary antibodies -
Description
Rabbit polyclonal to pan Cadherin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Bird, Fish, Amphibian
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Immunogen
Synthetic peptide:
DYDYLNDWGPRFKKLADMYGGGDD
conjugated to KLH by a Glutaraldehyde linker, corresponding to C terminal amino acids 889-912 of Chicken pan Cadherin. -
General notes
This product is useful for the detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, and for demonstration of adherens type cell-cell junctions regardless of their cadherin type.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
Purity
Whole antiserum -
Primary antibody notes
This product is useful for the detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, and for demonstration of adherens type cell-cell junctions regardless of their cadherin type. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody (ab6529) This image is courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK
Immunofluorescent imaging of human cells (U2OS) with ab6529 confirms the specificity of this antibody. Pan cadherin antibody recognises predominantly membranous signal with some fainter cytoplasmic staining corresponding to soluble cadherin. No nuclear staining is seen.
IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour. Primary antibody used at 1/100 in 5% milk / 0.2% TWEEN for one hour, secondary antibody for 30 minutes. All blocking and incubation steps carried out at 37 degrees C. Nuclei were stained with Hoechst stain.
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Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody (ab6529) This image is courtesy of an anonymous abreview.ab6529 staining pan Cadherin in human bone marrow cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed and blocked in 2% BSA for 30 minutes at 20°C. The primary antibody was diluted, 1/200 and incubated with sample for 9 hour at 4°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG, diluted 1/200 was used as secondary.
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Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody (ab6529) Image courtesy of Dr Hang Zhao by Abreview.ab6529 staining pan Cadherin in murine liver cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in formaldehyde, permeabilised in 0.4% Triton X-100 and then blocked using 10% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/200 for 17 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 594 (red) used at a 1/1000 dilution. The other secondary antibody used was an Alexa Fluor® 488 (green) goat anti-mouse IgG for detection of a specific cytosolic protein.
