Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Integrin beta 7 antibody [EP5948] - BSA and Azide free (ab216688)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP5948] to Integrin beta 7 - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Integrin beta 7 antibody [EP5948] - BSA and Azide free
    See all Integrin beta 7 primary antibodies

  • Description

    Rabbit monoclonal [EP5948] to Integrin beta 7 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: IM-9 cell lysate. IP: IM-9 whole cell lysate.

  • General notes

    ab216688 is the carrier-free version of ab137058 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab216688 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Integrin beta 7 antibody [EP5948] - BSA and Azide free (ab216688)
    Western blot - Anti-Integrin beta 7 antibody [EP5948] - BSA and Azide free (ab216688)
    Anti-Integrin beta 7 antibody [EP5948] (ab137058) at 1/1000 dilution + IM-9 cell lysate at 10 µg

    Secondary
    HRP labeled Goat anti-Rabbit IgG at 1/2000 dilution

    Predicted band size: 87 kDa



    This data was developed using ab137058, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-Integrin beta 7 antibody [EP5948] - BSA and Azide free (ab216688)
    Immunoprecipitation - Anti-Integrin beta 7 antibody [EP5948] - BSA and Azide free (ab216688)

    This data was developed using ab137058, the same antibody clone in a different buffer formulation.
    purifiedab137058 at 1/50 dilution (2µg) immunoprecipitating Integrin beta 7 in IM-9 whole cell lysate.
    Lane 1 (input): IM-9 (Human multiple myeloma B Lymphoblast) whole cell lysate 10µg
    Lane 2 (+): ab137058 + IM-9 whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab137058 in IM-9 whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 100-120 kDa

  • Anti-Integrin beta 7 antibody [EP5948] - BSA and Azide free (ab216688)
    Anti-Integrin beta 7 antibody [EP5948] - BSA and Azide free (ab216688)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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