Anti-Integrin beta 7 antibody [EP5948] (ab137058)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP5948] to Integrin beta 7
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-Integrin beta 7 antibody [EP5948]
See all Integrin beta 7 primary antibodies -
Description
Rabbit monoclonal [EP5948] to Integrin beta 7 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human -
Immunogen
Synthetic peptide corresponding to amino acids from the N terminal of Human Integrin beta 7 (UniProt ID: P26010).
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Positive control
- WB: IM-9 cell lysate. IP: IM-9 whole cell lysate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP5948 -
Isotype
IgG -
Research areas
Images
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Anti-Integrin beta 7 antibody [EP5948] (ab137058) at 1/1000 dilution + IM-9 cell lysate at 10 µg
Secondary
HRP labeled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 87 kDa
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purifiedab137058 at 1/50 dilution (2µg) immunoprecipitating Integrin beta 7 in IM-9 whole cell lysate.
Lane 1 (input): IM-9 (Human multiple myeloma B Lymphoblast) whole cell lysate 10µg
Lane 2 (+): ab137058 + IM-9 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab137058 in IM-9 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 100-120 kDa -