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Anti-HCN3 antibody [N141/28] (ab84818)

Key features and details

  • Mouse monoclonal [N141/28] to HCN3
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-HCN3 antibody [N141/28]
    See all HCN3 primary antibodies

  • Description

    Mouse monoclonal [N141/28] to HCN3

  • Host species

    Mouse

  • Specificity

    Does not cross react with other HCNs.

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Fusion protein corresponding to Mouse HCN3 aa 660-779 (C terminal).
    Database link: O88705

  • Positive control

    • Rat brain lysate.

  • General notes

    The clone number has been updated from S141-28 to N141/28, both clone numbers name the same antibody clone.

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-HCN3 antibody [N141/28] (ab84818)
    Western blot - Anti-HCN3 antibody [N141/28] (ab84818)
    Anti-HCN3 antibody [N141/28] (ab84818) at 1/1000 dilution + Rat brain membrane lysate

    Predicted band size: 87 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN3 antibody [N141/28] (ab84818)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN3 antibody [N141/28] (ab84818)
    ab84818 staining HCN3 in human hippocampal tissue by IHC-P (Bouin's fixed paraffin embedded).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN3 antibody [N141/28] (ab84818)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN3 antibody [N141/28] (ab84818)

    Ab84818 staining HCN3 in Mouse brain tissue sections by Immunohistochemistry. Samples were fixed with 10% Formalin solution at room temperature. Samples were incubated with primary antibody at 1/1000 dilution for 1 hour at room temperature. A HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase was used as the secondary antibody. Mayer Hematoxylin (blue) was used as nuclear counterstain. 

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