Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Wednesday March 03, 2021

Anti-HCN3 antibody [EPR13494(2)] (ab192025)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13494(2)] to HCN3
Suitable for: WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-HCN3 antibody [EPR13494(2)]
See all HCN3 primary antibodies
Description

Rabbit monoclonal [EPR13494(2)] to HCN3
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human cerebellum, fetal brain, forebrain and hypothalamus lysates; Mouse and Rat brain tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR13494(2)
Isotype

IgG
Research areas

Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)

All lanes : Anti-HCN3 antibody [EPR13494(2)] (ab192025) at 0.511 µg/ml

Lane 1 : Mouse Brain Tissue
Lane 2 : Rat Brain Tissue

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml

Predicted band size: 86 kDa
Observed band size: 100 kDa
why is the actual band size different from the predicted?

Blocking/Diluting Buffer and concentration: 5% NFDM /TBST
Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)

All lanes : Anti-HCN3 antibody [EPR13494(2)] (ab192025) at 1/10000 dilution

Lane 1 : Human cerebellum lysate
Lane 2 : Human fetal brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 86 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?

Blocking/dilution buffer: 5% NFDM/TBST.
Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)

All lanes : Anti-HCN3 antibody [EPR13494(2)] (ab192025) at 1/1000 dilution

Lane 1 : Human forebrain lysate
Lane 2 : Human hypothalamus lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 86 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?

Blocking/dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-HCN3 antibody [EPR13494(2)] (ab192025)

Western blot analysis of HCN3 in Human fetal brain lysate immunoprecipitated using ab192025 at 1/30 dilution.
Secondary antibody: Goat anti-Rabbit IgG (H+L), Peroxidase conjugated at 1/1000 dilution.
Blocking/dilution buffer: 5% NFDM/TBST.
Anti-HCN3 antibody [EPR13494(2)] (ab192025)

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