Antibodies, Proteins, Kits and Reagents for Life Science

338 390 ₸ Product size

100 µl 338 390 ₸

Order now and get it on Tuesday March 09, 2021

Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5517(2)] to HDAC1
Suitable for: WB, IHC-P, IP
Knockout validated
Reacts with: Human

Product name

Anti-HDAC1 antibody [EPR5517(2)]
See all HDAC1 primary antibodies
Description

Rabbit monoclonal [EPR5517(2)] to HDAC1
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human HDAC1 aa 1-100 (N terminal). The exact sequence is proprietary.
Positive control
- K562, Jurkat, MCF-7 and HeLa cell lysates; Human testis tissue; MCF-7 cells. IP: Jurkat whole cell lysate.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR5517(2)
Isotype

IgG
Research areas

Western blot - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: HDAC1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Human breast carcinoma lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab150399 observed at 65 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab150399 was shown to recognize HDAC1 when HDAC1 knockout samples were used, along with additional cross-reactive bands. Wild-type and HDAC1 knockout samples were subjected to SDS-PAGE. ab150399 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)
secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
Western blot - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

All lanes : Anti-HDAC1 antibody [EPR5517(2)] (ab150399) at 1/1000 dilution

Lane 1 : K562 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 55 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling HDAC1 with ab150399 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

Purified ab150399 at 1/30 dilution (2µg) immunoprecipitating HDAC1 in Jurkat whole cell lysate.
Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
Lane 2 (+): ab150399 + Jurkat whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab150399 in Jurkat whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 62 kDa
Faint band above 62kDa could be Sumoylated HDAC1. (PMID: 28186506)
Western blot - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: HDAC1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Human breast carcinoma lysate (20 µg) or K562 lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab150399 observed at 65 kDa. Red - loading control, ab8245, observed at 37 kDa.

This western blot image is a comparison between ab150399 and a competitor's top cited rabbit polyclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab150399 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

Immunohistochemical analysis of paraffin embedded normal Human stomach tissue using ab150399 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab150399 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab150399 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

Immunohistochemical analysis of paraffin embedded Human Thyroid gland carcinoma tissue using ab150399 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-HDAC1 antibody [EPR5517(2)] (ab150399)

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