Anti-HDAC1 antibody (ab53091)
Key features and details
- Rabbit polyclonal to HDAC1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-HDAC1 antibody
See all HDAC1 primary antibodies -
Description
Rabbit polyclonal to HDAC1 -
Host species
Rabbit -
Specificity
ab53091 detects endogenous levels of total HDAC1 protein. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseHumanIHC-P HumanWB Mouse
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Immunogen
Synthetic peptide derived from human HDAC1
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab53091 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HDAC1 antibody (ab53091)All lanes : Anti-HDAC1 antibody (ab53091) at 1/300 dilution
Lane 1 : NIH/3T3 cell extracts
Lane 2 : NIH/3T3 cell extracts with blocking peptide
Predicted band size: 55 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
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Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 antibody (ab53091)ICC/IF image of ab53091 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53091, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody (ab53091)Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ab53091 at 1/50 dilution, in the presence (right) or absence (left) of blocking peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 antibody (ab53091) This image is courtesy of an abreview submitted by Dr Mahesh Shivananjappa.Immunocytochemistry/ Immunofluorescence analysis of mouse RAW 264.7 cells labeling HDAC1 with ab53091 at 1/150 dilution. The cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton-X100 in 2% BSA for 15min. Blocking of the cells was done with 2% BSA for 1 hour at 4°C, followed by staining with ab53091 at 1/150 in 2% BSA in PBS for 16 hours at 4°C. An Alexa Fluor® 488 conjugated chicken anti-rabbit IgG (H+L) secondary antibody was used at 1/1000 dilution.
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Western blot - Anti-HDAC1 antibody (ab53091)All lanes : Anti-HDAC1 antibody (ab53091)
Lane 1 : NIH/3T3 cell lysates
Lane 2 : NIH/3T3 cell lysates blocked with synthesized peptide
Predicted band size: 55 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody (ab53091)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labeling HDAC1 with ab53091. The image on the right is blocked with the synthesized peptide.
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Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 antibody (ab53091)Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 cells labeling HDAC1 with ab53091. The image on the right is blocked with the synthesized peptide.
