Antibodies, Proteins, Kits and Reagents for Life Science

345 091 ₸ Product size

100 µl 345 091 ₸

Order now and get it on Tuesday March 02, 2021

Anti-HDAC1 antibody [EPR460(2)] (ab109411)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR460(2)] to HDAC1
Suitable for: IHC-P, WB, Flow Cyt
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-HDAC1 antibody [EPR460(2)]
See all HDAC1 primary antibodies
Description

Rabbit monoclonal [EPR460(2)] to HDAC1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Jurkat, HeLa, K562, and MCF7 cell lysates. IHC-P: Human colon and tonsil tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR460(2)
Isotype

IgG
Research areas

Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: HDAC1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Human breast carcinoma lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109411 observed at 65 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109411 was shown to recognize HDAC1 when HDAC1 knockout samples were used, along with additional cross-reactive bands. Wild-type and HDAC1 knockout samples were subjected to SDS-PAGE. ab109411 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed withGoat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

Immunohistochemical analysis of HDAC1 in paraffin-embedded Human colon tissue using ab109411 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

Overlay histogram showing HeLa cells stained with ab109411 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab109441, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150081) at 1/4000 dilution for 30 min at 22°C.

Isotype control antibody (black line) was rabbit IgG (monoclonal) (ab172730, 0.1ug/1x10⁶ cells) used under the same conditions.

Acquisition of >5,000 events were collected using an 50 mW Blue laser (488nm) with 530/30 bandpass filter.
Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: HDAC1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Human breast carcinoma lysate (20 µg) or K562 lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109411 observed at 65 kDa. Red - loading control, ab8245, observed at 37 kDa.

This western blot image is a comparison between ab109411 and a competitor's top cited rabbit polyclonal antibody.
Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

All lanes : Anti-HDAC1 antibody [EPR460(2)] (ab109411) at 1/1000 dilution

Lane 1 : Mouse brain lysate, 20ug
Lane 2 : Mouse heart lysate, 20ug
Lane 3 : Mouse Kidney lysate, 20ug
Lane 4 : Mouse spleen lysate,20ug

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated(Pierce). at 1/1000 dilution

Predicted band size: 55 kDa
Observed band size: 62 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking and Diluting buffer: 5% NFDM /TBST
Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

All lanes : Anti-HDAC1 antibody [EPR460(2)] (ab109411) at 1/1000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lane 4 : MCF7 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 55 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

Immunohistochemical analysis of HDAC1 in paraffin-embedded Human tonsil tissue using ab109411 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-HDAC1 antibody [EPR460(2)] (ab109411)

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