Anti-HDAC1 antibody (ab7028)
Key features and details
- Rabbit polyclonal to HDAC1
- Suitable for: ICC, WB, IP
- Reacts with: Mouse, Rat, Human, Chinese hamster
- Isotype: IgG
Overview
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Product name
Anti-HDAC1 antibody
See all HDAC1 primary antibodies -
Description
Rabbit polyclonal to HDAC1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIP HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human HDAC1 aa 466-482 conjugated to Keyhole Limpet Haemocyanin (KLH).
Sequence:C-KEEKPEAKGVKEEVKLA
Database link: HDAC1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
Whole antiserum is fractionated and further purified by anion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HDAC1 antibody - ChIP Grade (ab7028)All lanes : Anti-HDAC1 antibody (ab7028) at 1/10000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) cell lysate
Lane 4 : CHO (Chinese hamster ovary cell line) cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 6 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate
Secondary
All lanes : Goat Anti-Rabbit HRP
Predicted band size: 55 kDa
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Immunocytochemistry - Anti-HDAC1 antibody (ab7028) Hanigan, T.W. et al Send to PLoS One. 2017 Oct 18;12(10):e0186620. doi: 10.1371/journal.pone.0186620. eCollection 2017 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/MCF-7 cells were treated with indicated concentrations of panobinostat (optical sections A-D, respectively) or trichostatin A (optical sections E-H, respectively) for 12 hours, fixed, permeabilized and optical sections were obtained by laser scanning confocal microscopy. Fluorescence signal for HDAC1 is shown in green (left panels), DAPI staining is shown in blue (middle panels), and merged optical sections are shown in the right panels. Colocalization analysis of HDAC1 fluorescence signal and the DAPI stain signal was performed with JACoP (ImageJ).
HDAC1 was detected with ab7028. cells were ficed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
(After Figure 3 of Hanigan et al).
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Immunoprecipitation - Anti-HDAC1 antibody - ChIP Grade (ab7028)HDAC1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with ab7028.
Lane 1: 5 μl ab7028 in HeLa whole cell lysate.
Lane 2: 10 μl ab7028 in HeLa whole cell lysate.
Lane 3: 10 μl control antibody in HeLa whole cell lysate.
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Immunocytochemistry - Anti-HDAC1 antibody (ab7028)Immunofluorescence analysis of methanol fixed HeLa cells with ab7028 labelling HDAC1 at 1/200 dilution. The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.
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Western blot - Anti-HDAC1 antibody - ChIP Grade (ab7028) This image is courtesy of an anonymous AbreviewAll lanes : Anti-HDAC1 antibody (ab7028) at 1/1000 dilution
Lane 1 : Whole cell lysate from human HEK293 cell line
Lane 2 : Whole cell lysate from human HEK293 cell line treated with HDAC1 gene silencing shRNA
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit Ig at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Exposure time: 10 seconds
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Immunoprecipitation - Anti-HDAC1 antibody - ChIP Grade (ab7028)HDAC1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to HDAC1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab7028.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 60ka: HDAC1. -
Western blot - Anti-HDAC1 antibody - ChIP Grade (ab7028) This image is courtesy of an anonymous AbreviewAnti-HDAC1 antibody (ab7028) at 1/2000 dilution + Human HuH-7 whole cell lysate at 15 µg
Secondary
HRP conjugated Sheet anti-rabbit IgG polyclonal at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Exposure time: 20 seconds
Blocked with 5% milk for 1 hour at 20°C
