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Anti-Cytokeratin 10 antibody [SP99] - C-terminal (ab183317)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP99] to Cytokeratin 10 - C-terminal
  • Suitable for: IHC-Fr, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cytokeratin 10 antibody [SP99] - C-terminal
    See all Cytokeratin 10 primary antibodies

  • Description

    Rabbit monoclonal [SP99] to Cytokeratin 10 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    Rat
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human skin, skin squamous cell carcinoma, cervical squamous cell carcinoma tissues IHC-Fr: Rat and mouse skin tissue

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    Purified from TCS by protein A/G.

  • Clonality

    Monoclonal

  • Clone number

    SP99

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [SP99] - C-terminal (ab183317)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [SP99] - C-terminal (ab183317)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skin tissue sections labeling Cytokeratin 10 with ab183317 at 1/100 dilution (0.95 µg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 1 (pH 6.0) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human skin, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab183317 for 10 mins at room temperature.

  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 10 antibody [SP99] - C-terminal (ab183317)
    Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 10 antibody [SP99] - C-terminal (ab183317)

    Immunohistochemistry (Frozen) analysis of rat skin tissue section labeling Cytokeratin 10 with purified ab183317 at 1/50 (1.9 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 10 antibody [SP99] - C-terminal (ab183317)
    Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 10 antibody [SP99] - C-terminal (ab183317)

    Immunohistochemistry (Frozen) analysis of mouse skin tissue section labeling Cytokeratin 10 with purified ab183317 at 1/50 (1.9 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [SP99] - C-terminal (ab183317)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [SP99] - C-terminal (ab183317)

    Immunohistochemical analysis of Human skin labeling Cytokeratin 10 using ab183317 at a 1/100 dilution

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [SP99] - C-terminal (ab183317)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [SP99] - C-terminal (ab183317)

    Immunohistochemical analysis of Human skin labeling Cytokeratin 10 using ab183317 at a 1/100 dilution

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [SP99] - C-terminal (ab183317)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [SP99] - C-terminal (ab183317)

    Immunohistochemical analysis of Human skin squamous cell carcinoma labeling Cytokeratin 10 using ab183317 at a 1/100 dilution

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [SP99] - C-terminal (ab183317)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [SP99] - C-terminal (ab183317)

    Immunohistochemical analysis of Human cervical squamous cell carcinoma labeling Cytokeratin 10 using ab183317 at a 1/100 dilution.

  • Anti-Cytokeratin 10 antibody [SP99] - C-terminal (ab183317)
    Anti-Cytokeratin 10 antibody [SP99] - C-terminal (ab183317)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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