Anti-Cytokeratin 1 antibody (ab232685)
Key features and details
- Rabbit polyclonal to Cytokeratin 1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cytokeratin 1 antibody
See all Cytokeratin 1 primary antibodies -
Description
Rabbit polyclonal to Cytokeratin 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee
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Immunogen
Recombinant fragment (His-tag) corresponding to Human Cytokeratin 1 aa 450 to the C-terminus. (Expressed in E.coli).
Database link: P04264 -
Positive control
- IHC-P: Human kidney, breast cancer and liver cancer tissues. WB: A-375, NCI-H460 and HeLa cell lysates; Recombinant human Cytokeratin 1 protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab232685 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody (ab232685)
Paraffin-embedded human kidney tissue stained for Cytokeratin 1 using ab232685 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody (ab232685)Paraffin-embedded human breast cancer tissue stained for Cytokeratin 1 using ab232685 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody (ab232685)Paraffin-embedded human liver cancer tissue stained for Cytokeratin 1 using ab232685 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Western blot - Anti-Cytokeratin 1 antibody (ab232685)Anti-Cytokeratin 1 antibody (ab232685) at 2 µg/ml + Recombinant human Cytokeratin 1 protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 66 kDa
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Western blot - Anti-Cytokeratin 1 antibody (ab232685)All lanes : Anti-Cytokeratin 1 antibody (ab232685) at 2 µg/ml
Lane 1 : A-375 (human malignant melanoma cell line) cell lysate
Lane 2 : NCI-H460 (human lung cancer cell line) cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 66 kDa
