Anti-Cytokeratin 1 antibody [EPR17870] (ab185629)
![Anti-Cytokeratin 1 antibody [EPR17870] (ab185629)](https://www.abcam.com/ps/products/185/ab185629/Images/ab185629-237953-anti-cytokeratin-1-antibody-epr17870-immunohistochemistry.jpg "Anti-Cytokeratin 1 antibody [EPR17870] (ab185629)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17870] to Cytokeratin 1
- Suitable for: WB, IHC-P, IHC-Fr
- Reacts with: Mouse
Overview
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Product name
Anti-Cytokeratin 1 antibody [EPR17870]
See all Cytokeratin 1 primary antibodies -
Description
Rabbit monoclonal [EPR17870] to Cytokeratin 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-Fr MouseIHC-P MouseWB Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse skin lysate. IHC-P: Mouse skin tissue. IHC-Fr/IF: Mouse skin tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17870 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17870] (ab185629)
Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling Cytokeratin 1 with ab185629 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on keratinized epithelium of the mouse skin tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 1 antibody [EPR17870] (ab185629)](https://www.abcam.com/ps/products/185/ab185629/Images/ab185629-237948-anti-cytokeratin-1-antibody-epr17870-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 1 antibody [EPR17870] (ab185629)Immunohistochemical analysis of 4% paraformaldehyde-fixed frozen mouse skin tissue labeling Cytokeratin 1 with ab185629 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit (ab150077) secondary antibody at 1/500 dilution. Confocal image showing cytoplasm staining on keratinized epithelium of the mouse skin.
Negative control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit (ab150077) at 1/500 dilution.
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![Western blot - Anti-Cytokeratin 1 antibody [EPR17870] (ab185629)](https://www.abcam.com/ps/products/185/ab185629/Images/ab185629-237954-anti-cytokeratin-1-antibody-epr17870-western-blot.jpg)
Western blot - Anti-Cytokeratin 1 antibody [EPR17870] (ab185629)All lanes : Anti-Cytokeratin 1 antibody [EPR17870] (ab185629) at 1/5000 dilution
Lane 1 : Mouse skin lysates
Lane 2 : Mouse brain lysates
Lane 3 : Mouse heart lysates
Lane 4 : Mouse kidney lysates
Lane 5 : Mouse spleen lysates
Lane 6 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 66 kDa
Observed band size: 66 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Cytokeratin 1 is expressed in terminally differentiating epidermis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17870] (ab185629)](https://www.abcam.com/ps/products/185/ab185629/Images/ab185629-237952-anti-cytokeratin-1-antibody-epr17870-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17870] (ab185629)Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Cytokeratin 1 with ab185629 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Negative on mouse kidney tissue. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17870] (ab185629)](https://www.abcam.com/ps/products/185/ab185629/Images/ab185629-237951-anti-cytokeratin-1-antibody-epr17870-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17870] (ab185629)Immunohistochemical analysis of paraffin-embedded mouse brain tissue labeling Cytokeratin 1 with ab185629 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Negative on mouse brain tissue. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Anti-Cytokeratin 1 antibody [EPR17870] (ab185629)](https://www.abcam.com/ps/products/185/ab185629/Images/ab185629-6-benefits-of-recombinant-antibodies.png)
Anti-Cytokeratin 1 antibody [EPR17870] (ab185629)
