Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17744] to Cytokeratin 1
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cytokeratin 1 antibody [EPR17744]
    See all Cytokeratin 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR17744] to Cytokeratin 1

  • Host species

    Rabbit

  • Specificity

    Recombinant mouse cytokeratin 5 could be detected at very high antibody concentrations in WB.

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    IHC-P
    Mouse
    Rat
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse, rat and human skin lysates. IHC-P: Mouse and rat skin tissues. IHC-Fr: Mouse skin tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling Cytokeratin 1 with ab185628 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasm staining on mouse skin is observed (PMID: 12010363).

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Cytokeratin 1 with ab185628 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative Control: No staining on mouse stomach.

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)

    Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 1 with ab185628 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasm staining on rat skin is observed (PMID: 12010363).

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Cytokeratin 1 with ab185628 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative Control: No staining on rat kidney.

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)
    Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse skin tissue section labeling Cytokeratin 1 with ab185628 at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    The result showed cytoplasmic staining on mouse skin.

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Western blot - Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)
    Western blot - Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)
    All lanes : Anti-Cytokeratin 1 antibody [EPR17744] (ab185628) at 1/20000 dilution

    Lane 1 : Mouse skin lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Mouse spleen lysate
    Lane 4 : Mouse heart lysate
    Lane 5 : Rat skin lysate
    Lane 6 : Rat brain lysate
    Lane 7 : Rat spleen lysate
    Lane 8 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 9 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 66 kDa
    Observed band size: 66 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-4: 30 seconds; Lane 5-9: 3 minutes.

    Cytokeratin 1 is expressed in terminally differentiating epidermis (PMID: 2580302).

  • Western blot - Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)
    Western blot - Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)
    All lanes : Anti-Cytokeratin 1 antibody [EPR17744] (ab185628) at 1/20000 dilution

    Lane 1 : Human skin lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : Human fetal heart lysate
    Lane 4 : Human fetal kidney lysate
    Lane 5 : Human spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 66 kDa
    Observed band size: 66 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Cytokeratin 1 is expressed in terminally differentiating epidermis (PMID: 2580302).

  • Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)
    Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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