Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17744] to Cytokeratin 1
Suitable for: WB, IHC-P, IHC-Fr
Reacts with: Mouse, Rat, Human

Product name

Anti-Cytokeratin 1 antibody [EPR17744]
See all Cytokeratin 1 primary antibodies
Description

Rabbit monoclonal [EPR17744] to Cytokeratin 1
Host species

Rabbit
Specificity

Recombinant mouse cytokeratin 5 could be detected at very high antibody concentrations in WB.

Tested Applications & Species

Application	Species
IHC-Fr	Mouse
IHC-P	Mouse Rat
WB	Mouse Rat Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse, rat and human skin lysates. IHC-P: Mouse and rat skin tissues. IHC-Fr: Mouse skin tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17744
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)

Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling Cytokeratin 1 with ab185628 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm staining on mouse skin is observed (PMID: 12010363).

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Cytokeratin 1 with ab185628 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative Control: No staining on mouse stomach.

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)

Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 1 with ab185628 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm staining on rat skin is observed (PMID: 12010363).

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Cytokeratin 1 with ab185628 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative Control: No staining on rat kidney.

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse skin tissue section labeling Cytokeratin 1 with ab185628 at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

The result showed cytoplasmic staining on mouse skin.

The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Western blot - Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)

All lanes : Anti-Cytokeratin 1 antibody [EPR17744] (ab185628) at 1/20000 dilution

Lane 1 : Mouse skin lysate
Lane 2 : Mouse brain lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Mouse heart lysate
Lane 5 : Rat skin lysate
Lane 6 : Rat brain lysate
Lane 7 : Rat spleen lysate
Lane 8 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 9 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 66 kDa
Observed band size: 66 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1-4: 30 seconds; Lane 5-9: 3 minutes.

Cytokeratin 1 is expressed in terminally differentiating epidermis (PMID: 2580302).
Western blot - Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)

All lanes : Anti-Cytokeratin 1 antibody [EPR17744] (ab185628) at 1/20000 dilution

Lane 1 : Human skin lysate
Lane 2 : Human fetal brain lysate
Lane 3 : Human fetal heart lysate
Lane 4 : Human fetal kidney lysate
Lane 5 : Human spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 66 kDa
Observed band size: 66 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

Cytokeratin 1 is expressed in terminally differentiating epidermis (PMID: 2580302).
Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)

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