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Anti-CSF-1-R antibody [EPR20754] - BSA and Azide free (ab231074)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20754] to CSF-1-R - BSA and Azide free
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-CSF-1-R antibody [EPR20754] - BSA and Azide free
    See all CSF-1-R primary antibodies

  • Description

    Rabbit monoclonal [EPR20754] to CSF-1-R - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human lung cancer tissue. IP: Human tonsil tissue lysate.

  • General notes

    Ab231074 is the carrier-free version of ab229188. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab231074 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR20754] - BSA and Azide free (ab231074)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR20754] - BSA and Azide free (ab231074)

    Immunohistochemical analysis of paraffin-embedded human non-Hodgkin lymphoma tissue labeling CSF-1-R with ab229188 at 1/250 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Cytoplasmic and membranous staining of human non-Hodgkin lymphoma cells is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229188).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR20754] - BSA and Azide free (ab231074)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR20754] - BSA and Azide free (ab231074)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CSF-1-R with ab229188 at 1/250 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Cytoplasmic staining in macrophages of human tonsil (PMID: 26066800) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229188).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-CSF-1-R antibody [EPR20754] - BSA and Azide free (ab231074)
    Immunoprecipitation - Anti-CSF-1-R antibody [EPR20754] - BSA and Azide free (ab231074)

    CSF-1-R was immunoprecipitated from 0.35 mg of human tonsil tissue lysate with ab229188 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229188 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Human tonsil lysate 10 µg (Input). 
    Lane 2: ab229188 IP in human tonsil lysate. 
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229188 in human tonsil lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229188).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR20754] - BSA and Azide free (ab231074)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR20754] - BSA and Azide free (ab231074)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling CSF-1-R with ab229188 at 1/250 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Cytoplasmic staining in macrophages infiltrating human lung cancer. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229188).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-CSF-1-R antibody [EPR20754] - BSA and Azide free (ab231074)
    Anti-CSF-1-R antibody [EPR20754] - BSA and Azide free (ab231074)

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