Anti-CSF-1-R antibody (ab223642)
Key features and details
- Goat polyclonal to CSF-1-R
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CSF-1-R antibody
See all CSF-1-R primary antibodies -
Description
Goat polyclonal to CSF-1-R -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human CSF-1-R aa 700-800 (internal sequence) (Cysteine residue). (NP_005202.2).
Database link: P07333 -
Positive control
- WB: HEK-293 lysate overexpressing human CSF-1-R with C-terminal MYC tag.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab223642 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CSF-1-R antibody (ab223642)Lane 1 : Anti-CSF-1-R antibody (ab223642) at 0.5 µg/ml
Lane 2 : Anti-CSF-1-R antibody (ab223642) at 1 mg/ml
Lane 3 : Anti-MYC tag at 1/1000 dilution
Lanes 1 & 3 : HEK-293 (human epithelial cell line from embryonic kidney) lysate overexpressing human CSF-1-R with C-terminal MYC tag (in RIPA buffer)
Lane 2 : Mock transfected HEK-293 (human epithelial cell line from embryonic kidney) lysate (in RIPA buffer)
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 108 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?Primary incubations were for 1 hour.
