Anti-KMT6 / EZH2 antibody (ab228697)
Key features and details
- Rabbit polyclonal to KMT6 / EZH2
- Suitable for: WB, ChIP, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KMT6 / EZH2 antibody
See all KMT6 / EZH2 primary antibodies -
Description
Rabbit polyclonal to KMT6 / EZH2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ChIP, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Xenopus laevis, Zebrafish, Rhesus monkey
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Immunogen
Recombinant fragment within Human KMT6/ EZH2 (internal sequence). The exact sequence is proprietary.
Database link: Q15910 -
Positive control
- WB: NCI-H1299, HCT 116 and MCF7 whole cell lysates. ICC/IF: HeLa cells. ChIP: HeLa chromatin extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-KMT6 / EZH2 antibody (ab228697)All lanes : Anti-KMT6 / EZH2 antibody (ab228697) at 1/1000 dilution
Lane 1 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 85 kDa7.5% SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-KMT6 / EZH2 antibody (ab228697)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for KMT6 / EZH2 (green) using ab228697 at 1/200 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Nuclear counterstain: Hoechst 33342 (blue).
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ChIP - Anti-KMT6 / EZH2 antibody (ab228697)Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or ab228697. The precipitated DNA was detected by PCR with primer set targeting to CCND2.
