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Anti-KMT6 / EZH2 antibody [EPR9307(2)] - BSA and Azide free (ab231165)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9307(2)] to KMT6 / EZH2 - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, WB, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-KMT6 / EZH2 antibody [EPR9307(2)] - BSA and Azide free
    See all KMT6 / EZH2 primary antibodies

  • Description

    Rabbit monoclonal [EPR9307(2)] to KMT6 / EZH2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human breast carcinoma tissue; HeLa cells.

  • General notes

    Ab231165 is the carrier-free version of ab191080. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab231165 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Images

  • Western blot - Anti-KMT6 / EZH2 antibody [EPR9307(2)] - BSA and Azide free (ab231165)
    Western blot - Anti-KMT6 / EZH2 antibody [EPR9307(2)] - BSA and Azide free (ab231165)

    This WB data was generated using the same anti-KMT6/EZH2 antibody clone, EPR9307(2), in a different buffer formulation (cat# ab191080).

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: KMT6 / EZH2 knockout HAP1 cell lysate (20 µg) 
    Lane 3: HEK293 cell lysate (20 µg)
    Lane 4: Ms testis tissue lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab191080 observed at 93 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab191080 was shown to recognize KMT6 / EZH2 when KMT6 / EZH2 knockout samples were used, along with additional cross-reactive bands. Wild-type and KMT6 / EZH2 knockout samples were subjected to SDS-PAGE. ab191080 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Flow Cytometry - Anti-KMT6 / EZH2 antibody [EPR9307(2)] - BSA and Azide free (ab231165)
    Flow Cytometry - Anti-KMT6 / EZH2 antibody [EPR9307(2)] - BSA and Azide free (ab231165)

    ab191080 staining KMT6 / EZH2 in the human cell line Jurkat (human acute T cell leukemia) by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/90. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191080).

  • Immunocytochemistry/ Immunofluorescence - Anti-KMT6 / EZH2 antibody [EPR9307(2)] - BSA and Azide free (ab231165)
    Immunocytochemistry/ Immunofluorescence - Anti-KMT6 / EZH2 antibody [EPR9307(2)] - BSA and Azide free (ab231165)

    Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed; 0.1% tritonX-100-permeabilized) labeling KMT6 / EZH2 with ab191080 at 1/250 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue). 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191080).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6 / EZH2 antibody [EPR9307(2)] - BSA and Azide free (ab231165)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6 / EZH2 antibody [EPR9307(2)] - BSA and Azide free (ab231165)

    This IHC data was generated using the same anti-KMT6/EZH2 antibody clone, EPR9307(2), in a different buffer formulation (cat# ab191080).

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling KMT6 / EZH2 with ab191080 at 1/250 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin (inset: negative control).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-KMT6 / EZH2 antibody [EPR9307(2)] - BSA and Azide free (ab231165)
    Anti-KMT6 / EZH2 antibody [EPR9307(2)] - BSA and Azide free (ab231165)

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