Anti-KDM3A / JHDM2A antibody (ab229454)
Key features and details
- Rabbit polyclonal to KDM3A / JHDM2A
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KDM3A / JHDM2A antibody
See all KDM3A / JHDM2A primary antibodies -
Description
Rabbit polyclonal to KDM3A / JHDM2A -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Recombinant fragment within Human KDM3A/ JHDM2A (internal sequence). The exact sequence is proprietary.
Database link: Q9Y4C1 -
Positive control
- WB: HEK-293T and HepG2 whole cell extracts. IP: HEK-293T whole cell extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-KDM3A / JHDM2A antibody (ab229454)All lanes : Anti-KDM3A / JHDM2A antibody (ab229454) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 147 kDa5% SDS-PAGE gel.
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Immunoprecipitation - Anti-KDM3A / JHDM2A antibody (ab229454)KDM3A / JHDM2A was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 4 μg ab229454. Western blot was performed from the immunoprecipitate using ab229454 at 1/500 dilution.
Lane 1: HEK-293T whole cell extract 50 μg.
Lane 2: Control IP with preimmune Rabbit IgG instead of ab229454 in HEK-293T whole cell extract.
Lane 3: ab229454 IP in HEK-293T whole cell extract.
5 % SDS-PAGE gel.