Anti-KMT6 / EZH2 antibody (ab186006)
Key features and details
- Rabbit polyclonal to KMT6 / EZH2
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-KMT6 / EZH2 antibody
See all KMT6 / EZH2 primary antibodies -
Description
Rabbit polyclonal to KMT6 / EZH2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Xenopus tropicalis
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Immunogen
Synthetic peptide within Human KMT6/ EZH2 aa 650 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. CAA64955.1
Database link: Q15910 -
Positive control
- 293T, HeLa, Jurkat, and Mouse TCMK-1 whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-KMT6 / EZH2 antibody (ab186006)Lane 1 : Anti-KMT6 / EZH2 antibody (ab186006) at 1 µg
Lanes 2-4 : Anti-KMT6 / EZH2 antibody (ab186006) at 1 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : Mouse TCMK-1 cells.lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 85 kDa
Exposure time: 3 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6 / EZH2 antibody (ab186006)Mouse plasmacytoma tissue stained with ab186006 at a diltution of 1:1000 to detect EZH2.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6 / EZH2 antibody (ab186006)Human ovarian tissue stained with ab186006 at a diltution of 1:1000 to detect EZH2.
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Immunoprecipitation - Anti-KMT6 / EZH2 antibody (ab186006)Detection of KMT6 / EZH2 by Western Blot of Immunprecipitate.
Lane 1: ab186006 at 1 µg/ml labeling KMT6 / EZH2 in 293T whole cell lysate immunoprecipitated using ab186006 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with an exposure time of 3 minutes.
