Anti-KMT6 / EZH2 antibody [EPR9307] (ab150433)
![Anti-KMT6 / EZH2 antibody [EPR9307] (ab150433)](https://www.abcam.com/ps/products/150/ab150433/Images/ab150433-271628-anti-kmt6-ezh2-antibody-epr9307-western-blot.jpg "Anti-KMT6 / EZH2 antibody [EPR9307] (ab150433)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9307] to KMT6 / EZH2
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-KMT6 / EZH2 antibody [EPR9307]
See all KMT6 / EZH2 primary antibodies -
Description
Rabbit monoclonal [EPR9307] to KMT6 / EZH2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment within Human KMT6/ EZH2 aa 150-300. The exact sequence is proprietary.
Database link: Q15910 -
Positive control
- Recombinant Human KMT6 / EZH2 protein (ab132934) can be used as a positive control in WB. MCF7, Jurkat and HeLa cell lysates.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9307 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-KMT6 / EZH2 antibody [EPR9307] (ab150433)
Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: KMT6 / EZH2 knockout HAP1 cell lysate (40 µg)
Lane 3: HEK293 cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab150433 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab150433 was shown to recognize KMT6 / EZH2 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when KMT6 / EZH2 knockout samples were examined. Wild-type and KMT6 / EZH2 knockout samples were subjected to SDS-PAGE. Ab150433 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10,000 dilution respectively and incubated overnight at 4oC. Blots were developed with IRDye® 800CW Goat anti-Rabbit IgG (H + L) (ab216773)
and IRDye® 680 Goat anti-Mouse IgG (H + L) (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-KMT6 / EZH2 antibody [EPR9307] (ab150433)](https://www.abcam.com/ps/products/150/ab150433/Images/ab150433-157112-anti-kmt6-ezh2-antibody-epr9307-western-blot.jpg)
Western blot - Anti-KMT6 / EZH2 antibody [EPR9307] (ab150433)All lanes : Anti-KMT6 / EZH2 antibody [EPR9307] (ab150433) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 85 kDa
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![Anti-KMT6 / EZH2 antibody [EPR9307] (ab150433)](https://www.abcam.com/ps/products/150/ab150433/Images/ab150433-3-benefits-of-recombinant-antibodies.png)
Anti-KMT6 / EZH2 antibody [EPR9307] (ab150433)
