Anti-KMT6 / EZH2 antibody - ChIP Grade (ab195409)
Key features and details
- Rabbit polyclonal to KMT6 / EZH2 - ChIP Grade
- Suitable for: ChIP, WB, ChIP-sequencing
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KMT6 / EZH2 antibody - ChIP Grade
See all KMT6 / EZH2 primary antibodies -
Description
Rabbit polyclonal to KMT6 / EZH2 - ChIP Grade -
Host species
Rabbit -
Tested applications
Suitable for: ChIP, WB, ChIP-sequencingmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cynomolgus monkey
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Immunogen
Recombinant fragment corresponding to Mouse KMT6/ EZH2 aa 1-350.
Database link: Q61188 -
Positive control
- Chromatin prepared from K562 cells; HeLa nuclear extract.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.05% Sodium azide, 0.05% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ChIP - Anti-KMT6 / EZH2 antibody - ChIP Grade (ab195409)
ChIP results obtained with ab195409 directed against KMT6 / EZH2.
ChIP assays were performed using K562 cells, ab195409 and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. Quantitative PCR was performed with primers for MYT1 and HOXA9, used as positive control targets, and for the coding regions of the active CCT5 and EIF2S3 genes, used as negative controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
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ChIP-sequencing - Anti-KMT6 / EZH2 antibody - ChIP Grade (ab195409)ChIPseq results obtained with ab195409 directed against KMT6 / EZH2.
ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of ab195409. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. The figure shows the peak distribution along the short arm and a 6 Mb region containing several enriched regions of human chromosome 3 (figure A and B, respectively), and in two genomic regions containing the MYT1 gene on chromosome 20 and the HOX cluster on chromosome 7 (figure C and D).
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Western blot - Anti-KMT6 / EZH2 antibody - ChIP Grade (ab195409)Anti-KMT6 / EZH2 antibody - ChIP Grade (ab195409) at 1/1000 dilution + HeLa nuclear extract at 40 µg
Predicted band size: 85 kDaThe antiody was diluted in TBS-Tween containing 5% skimmed milk.
