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Anti-Jarid2 antibody [EPR6357(2)] - ChIP Grade (ab192252)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6357(2)] to Jarid2 - ChIP Grade
  • Suitable for: WB, ICC/IF, ChIP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Jarid2 antibody [EPR6357(2)] - ChIP Grade
    See all Jarid2 primary antibodies

  • Description

    Rabbit monoclonal [EPR6357(2)] to Jarid2 - ChIP Grade

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ChIP
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • NCCIT, SH-SY5Y, 293 and U87-MG cell lysates; SH-SY5Y cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Jarid2 antibody [EPR6357(2)] - ChIP Grade (ab192252)
    Western blot - Anti-Jarid2 antibody [EPR6357(2)] - ChIP Grade (ab192252)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)              
    Lane 2: JARID2 (KO) knockout  HAP1 whole cell lysate (20 µg)
    Lane 3: SH-SY5Y whole cell lysate (20 µg)
    Lane 4: U87MG whole cell lysate (20 µg)              

    Lanes 1 - 4: Merged signal (red and green). Green - ab192252 observed at 138 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab192252 was shown to specifically recognize JARID2 in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when JARID2 knockout samples were examined. Wild-type and JARID2 knockout samples were subjected to SDS-PAGE.  Ab192252 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Jarid2 antibody [EPR6357(2)] - ChIP Grade (ab192252)
    Immunocytochemistry/ Immunofluorescence - Anti-Jarid2 antibody [EPR6357(2)] - ChIP Grade (ab192252)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% triton X-100 permeabilized SH-SY5Y cells labeling Jarid2 with ab192252 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody (ab150078) at 1/500 dilution. Nuclear counter stain Dapi (blue).

     

    The two negative controls are ab192252 at 1/100 dilution followed by Goat anti mouse IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.

  • ChIP - Anti-Jarid2 antibody [EPR6357(2)] - ChIP Grade (ab192252)
    ChIP - Anti-Jarid2 antibody [EPR6357(2)] - ChIP Grade (ab192252)
    Chromatin was prepared from HeLa cells according to the Abcam Dual X-ChIP protocol*. Cells were fixed with EGS for 30 minutes, then formaldehyde for 10 minutes.
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab192252 (red), and 20 µl of Protein A/G sepharose beads. 5 µg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
    Primers and probes are located in the first kb of the transcribed region.
    *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol
  • Western blot - Anti-Jarid2 antibody [EPR6357(2)] - ChIP Grade (ab192252)
    Western blot - Anti-Jarid2 antibody [EPR6357(2)] - ChIP Grade (ab192252)
    All lanes : Anti-Jarid2 antibody [EPR6357(2)] - ChIP Grade (ab192252) at 1/10000 dilution

    Lane 1 : NCCIT cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 139 kDa
    Observed band size: 139 kDa



    Blocking/Dilution buffer: 5% NFDM /TBST.

  • Western blot - Anti-Jarid2 antibody [EPR6357(2)] - ChIP Grade (ab192252)
    Western blot - Anti-Jarid2 antibody [EPR6357(2)] - ChIP Grade (ab192252)
    Anti-Jarid2 antibody [EPR6357(2)] - ChIP Grade (ab192252) at 1/1000 dilution + U87-MG cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 139 kDa
    Observed band size: 139 kDa



    Blocking/Dilution buffer: 5% NFDM /TBST.

  • Anti-Jarid2 antibody [EPR6357(2)] - ChIP Grade (ab192252)
    Anti-Jarid2 antibody [EPR6357(2)] - ChIP Grade (ab192252)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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