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Epigenetics and Nuclear Signaling Chromosome Structure Chromosome

Anti-KIFAP3 antibody [EPR5600] (ab133537)

Price and availability

251 280 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-KIFAP3 antibody [EPR5600] (ab133537)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5600] to KIFAP3
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human

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Overview

  • Product name

    Anti-KIFAP3 antibody [EPR5600]
    See all KIFAP3 primary antibodies
  • Description

    Rabbit monoclonal [EPR5600] to KIFAP3
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human KIFAP3 aa 700-800. The exact sequence is proprietary.

  • Positive control

    • HeLa, SH-SY5Y, C6, and 293T cell lysates; and Human kidney tissue.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.05 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5600
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromosome Structure
    • Chromosome
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding

Images

  • Western blot - Anti-KIFAP3 antibody [EPR5600] (ab133537)
    Western blot - Anti-KIFAP3 antibody [EPR5600] (ab133537)
    All lanes : Anti-KIFAP3 antibody [EPR5600] (ab133537) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : C6 cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 91 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIFAP3 antibody [EPR5600] (ab133537)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIFAP3 antibody [EPR5600] (ab133537)

    Immunohistochemistry analysis of Paraffin Embedded Human kidney tissue labelling KIFAP3 with ab133537 at 1/50

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-KIFAP3 antibody [EPR5600] (ab133537)
    OI-RD Scanning - Anti-KIFAP3 antibody [EPR5600] (ab133537)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-KIFAP3 antibody [EPR5600] (ab133537)
    Anti-KIFAP3 antibody [EPR5600] (ab133537)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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