Anti-KIFAP3 antibody [EPR5600] (ab133537)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5600] to KIFAP3
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
Overview
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Product name
Anti-KIFAP3 antibody [EPR5600]
See all KIFAP3 primary antibodies -
Description
Rabbit monoclonal [EPR5600] to KIFAP3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide within Human KIFAP3 aa 700-800. The exact sequence is proprietary.
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Positive control
- HeLa, SH-SY5Y, C6, and 293T cell lysates; and Human kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.05 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5600 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-KIFAP3 antibody [EPR5600] (ab133537) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : C6 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 91 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
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Immunohistochemistry analysis of Paraffin Embedded Human kidney tissue labelling KIFAP3 with ab133537 at 1/50
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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