Anti-FMO3 antibody [EPR6967] (ab126790)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6967] to FMO3
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-FMO3 antibody [EPR6967]
See all FMO3 primary antibodies -
Description
Rabbit monoclonal [EPR6967] to FMO3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Rat -
Immunogen
A synthetic peptide corresponding to residues in Human FMO3 (UniProt P31513).
-
Positive control
- Rat kidney, Mouse liver, Rat liver and Human fetal liver lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6967 -
Isotype
IgG -
Research areas
Images
-
Overlay histogram showing HepG2 cells stained with ab126790 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126790, 1/65 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
-
All lanes : Anti-FMO3 antibody [EPR6967] (ab126790) at 1/1000 dilution
Lane 1 : Rat kidney lysate
Lane 2 : Mouse liver lysate
Lane 3 : Rat liver lysate
Lane 4 : Human fetal liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 60 kDa
-