Anti-Cathepsin S antibody [EPR5128] (ab134157)
![Anti-Cathepsin S antibody [EPR5128] (ab134157)](https://www.abcam.com/ps/products/134/ab134157/Images/ab134157-148438-anti-cathepsin-s-antibody-epr5128-western-blot.jpg "Anti-Cathepsin S antibody [EPR5128] (ab134157)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5128] to Cathepsin S
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Cathepsin S antibody [EPR5128]
See all Cathepsin S primary antibodies -
Description
Rabbit monoclonal [EPR5128] to Cathepsin S -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human Cathepsin S aa 300 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P25774 -
Positive control
- WB: Human spleen, U87-MG, and A549 cell lysates. IHC-P: Human kidney tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5128 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cathepsin S antibody [EPR5128] (ab134157)All lanes : Anti-Cathepsin S antibody [EPR5128] (ab134157) at 1/1000 dilution
Lane 1 : Human spleen lysate
Lane 2 : U87-MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 3 : A549 (Human lung carcinoma cell line) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 37 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin S antibody [EPR5128] (ab134157)](https://www.abcam.com/ps/products/134/ab134157/Images/ab134157-148437-anti-cathepsin-s-antibody-epr5128-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin S antibody [EPR5128] (ab134157)Immunohistochemical analysis of paraffin-embedded human kidney tissue labelling Cathepsin S with ab134157 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Cathepsin S antibody [EPR5128] (ab134157)](https://www.abcam.com/ps/products/134/ab134157/Images/ab134157-3-benefits-of-recombinant-antibodies.png)
Anti-Cathepsin S antibody [EPR5128] (ab134157)
