Anti-Cathepsin S antibody (ab92780)
Key features and details
- Rabbit polyclonal to Cathepsin S
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cathepsin S antibody
See all Cathepsin S primary antibodies -
Description
Rabbit polyclonal to Cathepsin S -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human Cathepsin S aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab101980) -
Positive control
- This antibody gave a positive signal in the following Human Tissue Lysates: Lung; Spleen; Thymus
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92780 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB HumanAll applications RatRabbitCowDogApplication Abreviews Notes WB Use a concentration of 1 mg/ml. Detects a band of approximately 37,25 kDa (predicted molecular weight: 37 kDa).ICC/IF Use a concentration of 5 µg/ml.Notes WB
Use a concentration of 1 mg/ml. Detects a band of approximately 37,25 kDa (predicted molecular weight: 37 kDa).ICC/IF
Use a concentration of 5 µg/ml.Target
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Function
Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N. -
Sequence similarities
Belongs to the peptidase C1 family. -
Cellular localization
Lysosome. - Information by UniProt
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Database links
- Entrez Gene: 327711 Cow
- Entrez Gene: 403400 Dog
- Entrez Gene: 1520 Human
- Entrez Gene: 50654 Rat
- Omim: 116845 Human
- SwissProt: P25326 Cow
- SwissProt: Q8HY81 Dog
- SwissProt: P25774 Human
see all -
Alternative names
- Cathepsin S antibody
- CathepsinS antibody
- CATS_HUMAN antibody
- Ctss antibody
Images
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Western blot - Anti-Cathepsin S antibody (ab92780)All lanes : Anti-Cathepsin S antibody (ab92780) at 1 µg/ml
Lane 1 : Lung (Human) Tissue Lysate
Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lane 3 : Human thymus tissue lysate - total protein (ab30146)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 37 kDa
Additional bands at: 19 kDa, 25 kDa (possible cleavage fragment). We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
We hypothesize that the 25 kDa band represents the mature form of Cathepsin S. Abcam welcomes customer feedback. -
Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin S antibody (ab92780)ICC/IF image of ab92780 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92780, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (3)
ab92780 has been referenced in 3 publications.
- Kim S et al. Regulating BRCA1 protein stability by cathepsin S-mediated ubiquitin degradation. Cell Death Differ N/A:N/A (2018). PubMed: 30006610
- Pires D et al. Mycobacterium tuberculosis Modulates miR-106b-5p to Control Cathepsin S Expression Resulting in Higher Pathogen Survival and Poor T-Cell Activation. Front Immunol 8:1819 (2017). PubMed: 29326705
- Wong WJ et al. MYC degradation under low O2 tension promotes survival by evading hypoxia-induced cell death. Mol Cell Biol 33:3494-504 (2013). PubMed: 23816886
Images
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Western blot - Anti-Cathepsin S antibody (ab92780)All lanes : Anti-Cathepsin S antibody (ab92780) at 1 µg/ml
Lane 1 : Lung (Human) Tissue Lysate
Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lane 3 : Human thymus tissue lysate - total protein (ab30146)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 37 kDa
Additional bands at: 19 kDa, 25 kDa (possible cleavage fragment). We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
We hypothesize that the 25 kDa band represents the mature form of Cathepsin S. Abcam welcomes customer feedback. -
Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin S antibody (ab92780)ICC/IF image of ab92780 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92780, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
