Anti-ErbB2 / HER2 antibody [SP101] (ab231438)
![Anti-ErbB2 / HER2 antibody [SP101] (ab231438)](https://www.abcam.com/ps/products/231/ab231438/Images/ab231438-2-AntiErbB-2-antibody-SP101-IHC-human-breast-carcinoma.jpg "Anti-ErbB2 / HER2 antibody [SP101] (ab231438)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP101] to ErbB2 / HER2
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
-
Product name
Anti-ErbB2 / HER2 antibody [SP101]
See all ErbB2 / HER2 primary antibodies -
Description
Rabbit monoclonal [SP101] to ErbB2 / HER2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
-
Immunogen
Recombinant fragment within Human ErbB2/ HER2 aa 500-650. The exact sequence is proprietary. Extracellular domain.
Database link: P04626 -
Positive control
- IHC-P: Human breast carcinoma tissue. WB: SK-BR-3 whole cell lysate.
-
General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
-
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from TCS by Protein A/G. -
Clonality
Monoclonal -
Clone number
SP101 -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [SP101] (ab231438)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling ErbB2 / HER2 with ab231438 at 1/100 dilution (6.38 µg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 1 (pH 6.0) for 10mins Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Membranous staining on the human breast carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab231438 for 30 mins at room temperature. -
![Western blot - Anti-ErbB2 / HER2 antibody [SP101] (ab231438)](https://www.abcam.com/ps/products/231/ab231438/Images/ab231438-366437-anti-erbb2-antibody-sp101-western-blot-skbr3-human.jpg)
Western blot - Anti-ErbB2 / HER2 antibody [SP101] (ab231438)Lane 1 : Anti-ErbB2 / HER2 antibody [SP101] (ab231438) at 0.638 µg/ml (purified)
Lane 2 : Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
All lanes : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 137 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking/Diluting Buffer and concentration: 5% NFDM /TBST
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [SP101] (ab231438)](https://www.abcam.com/ps/products/231/ab231438/Images/ab231438-301228-anti-erbb-2-antibody-sp101-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [SP101] (ab231438)Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for ErbB2 / HER2 using ab231438 at 1/100 dilution in immunohistochemical analysis.
-
![Anti-ErbB2 / HER2 antibody [SP101] (ab231438)](https://www.abcam.com/ps/products/231/ab231438/Images/ab231438-5-benefits-of-recombinant-antibodies.png)
Anti-ErbB2 / HER2 antibody [SP101] (ab231438)
