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Epigenetics and Nuclear Signaling Chromosome Structure Telomeres

Anti-TRF1 antibody [572C] (ab192629)

Price and availability

268 032 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-TRF1 antibody [572C] (ab192629)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rat monoclonal [572C] to TRF1
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG2a

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Overview

  • Product name

    Anti-TRF1 antibody [572C]
    See all TRF1 primary antibodies
  • Description

    Rat monoclonal [572C] to TRF1
  • Host species

    Rat
  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    Human
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length protein (His-tag) corresponding to Mouse TRF1 aa 1 to the C-terminus.
    Database link: P70371

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC: HEK-TRF1 and HEK-TRF2 transfected cell lines. WB: NIH-3T3 and Hek-TRF1 cell lysate, IPS-GFP-TRF1 homo cell lysate. ICC/IF: iPS cells,HRK-TRF1 and IPS-GFP-TRF1homo cell lines.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    HiTrap protein G columns
  • Clonality

    Monoclonal
  • Clone number

    572C
  • Isotype

    IgG2a
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromosome Structure
    • Telomeres

Images

  • Western blot - Anti-TRF1 [572C] antibody (ab192629)
    Western blot - Anti-TRF1 [572C] antibody (ab192629)
    All lanes : Anti-TRF1 antibody [572C] (ab192629)

    Lane 1 : IPS-GFP-TRF1 homo at 100 µg
    Lane 2 : IPS-GFP-TRF1 heter at 100 µg
    Lane 3 : IPS-sh TRF1 at 100 µg
    Lane 4 : IPS-sh TRF1 puro at 100 µg
    Lane 5 : HEK-TRF1 at 20 µg
    Lane 6 : HEK-FGFR2 at 20 µg
    Lane 7 : NIH-3T3 at 100 µg

    Predicted band size: 50 kDa



    ab192629 used undiluted for all lanes.

  • Immunocytochemistry/ Immunofluorescence - Anti-TRF1 [572C] antibody (ab192629)
    Immunocytochemistry/ Immunofluorescence - Anti-TRF1 [572C] antibody (ab192629)

    Immunocytochemistry/ Immunofluorescent analysis of HEK-TRF1, HEK-GFP-TRF2, IPS-GFP-TRF1homo and IPS-shTRF1homo cell lines labeling TRF1 with undiluted ab192629.

  • Immunocytochemistry/ Immunofluorescence - Anti-TRF1 [572C] antibody (ab192629)
    Immunocytochemistry/ Immunofluorescence - Anti-TRF1 [572C] antibody (ab192629)

    Immunocytochemistry/ Immunofluorescent analysis of frozen cytospins preparations of mTRF1 expressed in HEK293T labeling TRF1 using ab192629. Negative control on right used mTRF2-GFP expressed in HEK293T.

  • Western blot - Anti-TRF1 [572C] antibody (ab192629)
    Western blot - Anti-TRF1 [572C] antibody (ab192629)
    All lanes : Anti-TRF1 antibody [572C] (ab192629) at 1/500 dilution

    Lane 1 : Mefs TRF1 +/+ nuclear extract
    Lane 2 : Mefs TRF1 KO nuclear extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 50 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-TRF1 [572C] antibody (ab192629)
    Immunocytochemistry/ Immunofluorescence - Anti-TRF1 [572C] antibody (ab192629)

    Immunocytochemistry/ Immunofluorescent analysis of mouse blastocyte labeling TRF1 with ab192629 at a 1/100 dilution.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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