Anti-TRF1 antibody [572C] (ab192629)
Key features and details
- Rat monoclonal [572C] to TRF1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG2a
Overview
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Product name
Anti-TRF1 antibody [572C]
See all TRF1 primary antibodies -
Description
Rat monoclonal [572C] to TRF1 -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseHumanWB MouseHuman -
Immunogen
Recombinant full length protein (His-tag) corresponding to Mouse TRF1 aa 1 to the C-terminus.
Database link: P70371 -
Positive control
- IHC: HEK-TRF1 and HEK-TRF2 transfected cell lines. WB: NIH-3T3 and Hek-TRF1 cell lysate, IPS-GFP-TRF1 homo cell lysate. ICC/IF: iPS cells,HRK-TRF1 and IPS-GFP-TRF1homo cell lines.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
HiTrap protein G columns -
Clonality
Monoclonal -
Clone number
572C -
Isotype
IgG2a -
Research areas
Images
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All lanes : Anti-TRF1 antibody [572C] (ab192629)
Lane 1 : IPS-GFP-TRF1 homo at 100 µg
Lane 2 : IPS-GFP-TRF1 heter at 100 µg
Lane 3 : IPS-sh TRF1 at 100 µg
Lane 4 : IPS-sh TRF1 puro at 100 µg
Lane 5 : HEK-TRF1 at 20 µg
Lane 6 : HEK-FGFR2 at 20 µg
Lane 7 : NIH-3T3 at 100 µg
Predicted band size: 50 kDaab192629 used undiluted for all lanes.
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Immunocytochemistry/ Immunofluorescent analysis of HEK-TRF1, HEK-GFP-TRF2, IPS-GFP-TRF1homo and IPS-shTRF1homo cell lines labeling TRF1 with undiluted ab192629.
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Immunocytochemistry/ Immunofluorescent analysis of frozen cytospins preparations of mTRF1 expressed in HEK293T labeling TRF1 using ab192629. Negative control on right used mTRF2-GFP expressed in HEK293T.
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All lanes : Anti-TRF1 antibody [572C] (ab192629) at 1/500 dilution
Lane 1 : Mefs TRF1 +/+ nuclear extract
Lane 2 : Mefs TRF1 KO nuclear extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 50 kDa
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Immunocytochemistry/ Immunofluorescent analysis of mouse blastocyte labeling TRF1 with ab192629 at a 1/100 dilution.