Anti-TRF1 antibody (ab85871)
Key features and details
- Rabbit polyclonal to TRF1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRF1 antibody
See all TRF1 primary antibodies -
Description
Rabbit polyclonal to TRF1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human TRF1 aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab97492) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: Jurkat; HEK293; U20S; Caco2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85871 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications HamsterOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 50, 48 kDa (predicted molecular weight: 50 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50, 48 kDa (predicted molecular weight: 50 kDa).Target
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Function
Binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. -
Tissue specificity
Highly expressed and ubiquitous. Isoform Pin2 predominates. -
Sequence similarities
Contains 1 HTH myb-type DNA-binding domain. -
Domain
The acidic N-terminal domain binds to the ankyrin repeats of TNKS1 and TNKS2. The C-terminal domain binds microtubules.
The TRFH dimerization region mediates the interaction with TINF2. -
Post-translational
modificationsPhosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage.
ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA.
Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex, leading to its degradation by the proteasome. -
Cellular localization
Nucleus. Cytoplasm > cytoskeleton > spindle. Chromosome > telomere. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Associates with the mitotic spindle. - Information by UniProt
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Database links
- Entrez Gene: 7013 Human
- Omim: 600951 Human
- SwissProt: P54274 Human
- Unigene: 442707 Human
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Alternative names
- hTRF1 AS antibody
- NIMA interacting protein 2 antibody
- NIMA-interacting protein 2 antibody
see all
Images
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Western blot - Anti-TRF1 antibody (ab85871)All lanes : Anti-TRF1 antibody (ab85871) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 4 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 48 + 50 kDa why is the actual band size different from the predicted?
Additional bands at: 125 kDa, 20 kDa, 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
This antibody was raised against an immunogen that is predicted to recognize isoforms 1 and 2 of Human Telomeric repeat-binding factor 1 (TRF1). The molecular weights of isoforms 1 and 2 are predicted to be 50 kDa and 48 kDa, respectively.
Datasheets and documents
References (0)
ab85871 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-TRF1 antibody (ab85871)All lanes : Anti-TRF1 antibody (ab85871) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 4 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 48 + 50 kDa why is the actual band size different from the predicted?
Additional bands at: 125 kDa, 20 kDa, 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
This antibody was raised against an immunogen that is predicted to recognize isoforms 1 and 2 of Human Telomeric repeat-binding factor 1 (TRF1). The molecular weights of isoforms 1 and 2 are predicted to be 50 kDa and 48 kDa, respectively.
