Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

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Anti-TRAP220/MED1 antibody [BLR037F] (ab243893)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR037F] to TRAP220/MED1
Suitable for: WB, ICC, IHC-P, IP
Reacts with: Mouse, Human

Product name

Anti-TRAP220/MED1 antibody [BLR037F]
See all TRAP220/MED1 primary antibodies
Description

Rabbit monoclonal [BLR037F] to TRAP220/MED1
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human TRAP220/MED1 aa 1550-1581 (C terminal). The exact sequence is proprietary. NP_004765.2 and Gene ID 5469.
Database link: Q15648
General notes

This product is sold under License from Bethyl Laboratories, Inc.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR037F
Isotype

IgG
Research areas

Immunocytochemistry - Anti-TRAP220/MED1 antibody [BLR037F] (ab243893)

Formalin-fixed, paraffin-embedded HCT 116 (human colorectal carcinoma cell line) cells labeling TRAF220/MED1 using ab243893 at 1/100 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP220/MED1 antibody [BLR037F] (ab243893)

Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for TRAP220/MED1 using ab243893 at 1/500 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP220/MED1 antibody [BLR037F] (ab243893)

Formalin-fixed, paraffin-embedded human small cell lung cancer tissue stained for TRAP220/MED1using ab243893 at 1/500 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
Immunoprecipitation - Anti-TRAP220/MED1 antibody [BLR037F] (ab243893)

TRAP220/MED1 was immunoprecipitated from 1.0 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab243893 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243893 at 1/1000 dilution.
Lane 1: ab243893 IP in HeLa whole cell lysate.

Lane 2: Control IgG in HeLa whole cell lysate.

Detection: Chemiluminescence with an exposure time of 30 seconds.
Western blot - Anti-TRAP220/MED1 antibody [BLR037F] (ab243893)

Western blot analysis using ab243893 at 1/1000 dilution.
Lane 1: HEK-293T whole cell lysate (50 µg).
Lane 2: MCF7 whole cell lysate (50 µg).
Lane 3: HepG2 whole cell lysate (50 µg).
Lane 4: A549 whole cell lysate (50 µg).
Lane 5: SW 260 whole cell lysate (50 µg).
Lane 6: SK whole cell lysate (50 µg).
Lane 7: Jurkat whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 3 seconds.
Anti-TRAP220/MED1 antibody [BLR037F] (ab243893)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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