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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-TRAP220/MED1 antibody (ab64965)

Price and availability

328 339 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-TRAP220/MED1 antibody (ab64965)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to TRAP220/MED1
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-TRAP220/MED1 antibody
    See all TRAP220/MED1 primary antibodies
  • Description

    Rabbit polyclonal to TRAP220/MED1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from internal region of human TRAP220/MED1

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • Other
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Co-activators/co-repressors
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Types of disease
    • Cancer

Images

  • Western blot - Anti-TRAP220/MED1 antibody (ab64965)
    Western blot - Anti-TRAP220/MED1 antibody (ab64965)
    All lanes : Anti-TRAP220/MED1 antibody (ab64965) at 1/500 dilution

    Lane 1 : extracts from Jurkat cells, without immunising peptide
    Lane 2 : extracts from Jurkat cells, with immunising peptide

    Predicted band size: 180 kDa
    Observed band size: 170 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 110 kDa. We are unsure as to the identity of these extra bands.



    The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP220/MED1 antibody (ab64965)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP220/MED1 antibody (ab64965)
    Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using 1/50 ab64965, after antigen retrieval by boiling in a pressure cooker for 2-3 min. Polymer system was used for signal enhancing and DAB staining was performed for visualising the signal.
  • Western blot - Anti-TRAP220/MED1 antibody (ab64965)
    Western blot - Anti-TRAP220/MED1 antibody (ab64965)
    Anti-TRAP220/MED1 antibody (ab64965) at 1/1000 dilution + MEF (Mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

    Secondary
    Alexa Fluor 680 anti-Rabbit antibody at 1/5000 dilution

    Predicted band size: 180 kDa



    Exposure time: 10 minutes

  • Western blot - Anti-TRAP220/MED1 antibody (ab64965)
    Western blot - Anti-TRAP220/MED1 antibody (ab64965)
    Anti-TRAP220/MED1 antibody (ab64965) at 1/1000 dilution + U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate at 50 µg

    Secondary
    Alexa Fluor 680 anti-Rabbit

    Predicted band size: 180 kDa



    Exposure time: 10 minutes.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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