Anti-MCM7/PRL antibody (ab238529)
Key features and details
- Rabbit polyclonal to MCM7/PRL
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MCM7/PRL antibody
See all MCM7/PRL primary antibodies -
Description
Rabbit polyclonal to MCM7/PRL -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human MCM7/PRL aa 350 to the C-terminus. NP_005907.3
Database link: P33993-1 -
Positive control
- WB: A549, HeLa, HL-60, MCF7 and SW480 cell extracts. ICC/IF: HeLa cells.
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General notes
This product was previously labelled as MCM7
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MCM7/PRL antibody (ab238529)All lanes : Anti-MCM7/PRL antibody (ab238529) at 1/1000 dilution
Lane 1 : A549 (Human lung carcinoma cell line) cell extracts
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extracts
Lane 3 : HL-60 (Human promyelocytic leukemia cell line) cell extracts
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) cell extracts
Lane 5 : SW480 (Human colorectal adenocarcinoma cell line) cell extracts
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 81 kDa
Exposure time: 10 secondsBlocking buffer: 3% non-fat dry milk in TBST.
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Immunocytochemistry/ Immunofluorescence - Anti-MCM7/PRL antibody (ab238529)HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for MCM7/PRL using ab238529 (Top panel, green) at a 1/50 dilution in ICC/IF. Counterstained with DAPI (Bottom panel, merge).
