Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-nSMase antibody [EPR6718] (ab131330)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6718] to nSMase
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-nSMase antibody [EPR6718]
    See all nSMase primary antibodies

  • Description

    Rabbit monoclonal [EPR6718] to nSMase

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human nSMase aa 1-100. The exact sequence is proprietary.

  • Positive control

    • WB: 293T, MOLT4 and K562 cell lysates; U-87MG cells; Mouse brain and Rat brain lysates. IP: Mouse brain lysate

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunoprecipitation - Anti-nSMase antibody [EPR6718] (ab131330)
    Immunoprecipitation - Anti-nSMase antibody [EPR6718] (ab131330)

    ab131330 (purified) at 1:30 dilution (2µg) immunoprecipitating nSMase in Mouse brain lysate.
    Lane 1 (input): Mouse brain lysate 10µg
    Lane 2 (+): ab131330 & Mouse brain lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab131330 in Mouse brain lysate
    For western blotting, VeriBlot for IP Detection Reagent(HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-nSMase antibody [EPR6718] (ab131330)
    Western blot - Anti-nSMase antibody [EPR6718] (ab131330)
    All lanes : Anti-nSMase antibody [EPR6718] (ab131330) at 1/1000 dilution (Purified)

    Lane 1 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysates
    Lane 2 : Mouse brain lysates
    Lane 3 : Rat brain lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 48 kDa
    Observed band size: 48 kDa

  • Western blot - Anti-nSMase antibody [EPR6718] (ab131330)
    Western blot - Anti-nSMase antibody [EPR6718] (ab131330)
    All lanes : Anti-nSMase antibody [EPR6718] (ab131330) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : MOLT4 cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 48 kDa



    This image was generated using the unpurified version of the product. 

  • Western blot - Anti-nSMase antibody [EPR6718] (ab131330)
    Western blot - Anti-nSMase antibody [EPR6718] (ab131330)
    All lanes : Anti-nSMase antibody [EPR6718] (ab131330) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 48 kDa



    This image was generated using the unpurified version of the product. 

  • OI-RD Scanning - Anti-nSMase antibody [EPR6718] (ab131330)
    OI-RD Scanning - Anti-nSMase antibody [EPR6718] (ab131330)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-nSMase antibody [EPR6718] (ab131330)
    Anti-nSMase antibody [EPR6718] (ab131330)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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