Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Torsin A/DYT1 antibody [EP2569Y] - BSA and Azide free (ab247447)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2569Y] to Torsin A/DYT1 - BSA and Azide free
  • Suitable for: Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Torsin A/DYT1 antibody [EP2569Y] - BSA and Azide free
    See all Torsin A/DYT1 primary antibodies

  • Description

    Rabbit monoclonal [EP2569Y] to Torsin A/DYT1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: SW480, Caco-2, A549, NIH/3T3, and C6 whole cell lysates. Rat brain and mouse lung tissue lysates; Flow Cyt: SH-SY5Y cells.

  • General notes

    ab247447 is the carrier-free version of ab76133 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247447 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as Torsin A

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Torsin A/DYT1 antibody [EP2569Y] - BSA and Azide free (ab247447)
    Western blot - Anti-Torsin A/DYT1 antibody [EP2569Y] - BSA and Azide free (ab247447)
    All lanes : Anti-Torsin A/DYT1 antibody [EP2569Y] (ab76133) at 1/5000 dilution (purified)

    Lane 1 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : Rat brain lysate
    Lane 4 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
    Lane 5 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 6 : Mouse lung lysate
    Lane 7 : C6 (Rat glial tumor glial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 37 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76133).

  • Flow Cytometry - Anti-Torsin A/DYT1 antibody [EP2569Y] - BSA and Azide free (ab247447)
    Flow Cytometry - Anti-Torsin A/DYT1 antibody [EP2569Y] - BSA and Azide free (ab247447)

    Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Torsin A/DYT1 with purified ab76133 at 1/100 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS ,BSA, glycerol, and sodium azide (ab76133).

  • Anti-Torsin A/DYT1 antibody [EP2569Y] - BSA and Azide free (ab247447)
    Anti-Torsin A/DYT1 antibody [EP2569Y] - BSA and Azide free (ab247447)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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