Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Torsin A/DYT1 antibody (ab34540)

Key features and details

  • Rabbit polyclonal to Torsin A/DYT1
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Torsin A/DYT1 antibody
    See all Torsin A/DYT1 primary antibodies

  • Description

    Rabbit polyclonal to Torsin A/DYT1

  • Host species

    Rabbit

  • Specificity

    The immunogen used to raise this antibody has 92% homology with the human Torsin A/DYT1 protein. Some customers have successfully used ab34540 in human tissue. Please contact Abcam Scientific Support for more information.

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to Rat Torsin A/DYT1 aa 300 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab35510, ab35511)

  • General notes

     This product was previously labelled as Torsin A.

Properties

Applications

Our Abpromise guarantee covers the use of ab34540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).

Target

Images

  • Western blot - Anti-Torsin A/DYT1 antibody (ab34540)
    Western blot - Anti-Torsin A/DYT1 antibody (ab34540)
    All lanes : Anti-Torsin A/DYT1 antibody (ab34540) at 1 µg/ml

    Lane 1 : Marker
    Lane 2 : Mouse Brain at 10 µg
    Lane 3 : Testis (Mouse) Whole Cell Lysate - normal tissue at 10 µg
    Lane 4 : Mouse Heart Mouse Tissue Lysate at 10 µg
    Lane 5 : Mouse skeletal muscle tissue lysate - total protein (ab29711) at 10 µg
    Lane 6 : Mouse Fetal lysate - 14 Days old Tissue Lysate at 10 µg
    Lane 7 : Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg
    Lane 8 : Mouse Embryonic Fibroblast 1 at 10 µg
    Lane 9 : NIH/3T3 whole cell lysate (ab7179) at 10 µg

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size: 38 kDa
    Observed band size: 38 kDa
    Additional bands at: 105 kDa, 65 kDa. We are unsure as to the identity of these extra bands.

  • Western blot - Anti-Torsin A/DYT1 antibody (ab34540)
    Western blot - Anti-Torsin A/DYT1 antibody (ab34540)
    All lanes : Anti-Torsin A/DYT1 antibody (ab34540) at 1 µg/ml

    Lane 1 : NIH/3T3 whole cell lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Liver (Mouse) Tissue Lysate - normal tissue
    Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 5 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 38 kDa
    Observed band size: 38 kDa
    Additional bands at: 65 kDa, 65 kDa. We are unsure as to the identity of these extra bands.

  • Immunocytochemistry/ Immunofluorescence - Anti-Torsin A/DYT1 antibody (ab34540)
    Immunocytochemistry/ Immunofluorescence - Anti-Torsin A/DYT1 antibody (ab34540)
    ICC/IF image of ab34540 stained SHSY5Y cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab34540, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

References (27)

Publishing research using ab34540? Please let us know so that we can cite the reference in this datasheet.

ab34540 has been referenced in 27 publications.

  • Yokoi F  et al. Decreased number of striatal cholinergic interneurons and motor deficits in dopamine receptor 2-expressing-cell-specific Dyt1 conditional knockout mice. Neurobiol Dis 134:104638 (2020). WB ; Mouse . PubMed: 31618684
  • Yokoi F  et al. Improved survival and overt "dystonic" symptoms in a torsinA hypofunction mouse model. Behav Brain Res 381:112451 (2020). WB ; Mouse . PubMed: 31891745
  • Bonsi P  et al. RGS9-2 rescues dopamine D2 receptor levels and signaling in DYT1 dystonia mouse models. EMBO Mol Med 11:N/A (2019). PubMed: 30552094
  • Beauvais G  et al. Efficient RNA interference-based knockdown of mutant torsinA reveals reversibility of PERK-eIF2a pathway dysregulation in DYT1 transgenic rats in vivo. Brain Res 1706:24-31 (2019). PubMed: 30366018
  • Pappas SS  et al. A cell autonomous torsinA requirement for cholinergic neuron survival and motor control. Elife 7:N/A (2018). PubMed: 30117805
View all Publications for this product

Images

  • Western blot - Anti-Torsin A/DYT1 antibody (ab34540)
    Western blot - Anti-Torsin A/DYT1 antibody (ab34540)
    All lanes : Anti-Torsin A/DYT1 antibody (ab34540) at 1 µg/ml

    Lane 1 : Marker
    Lane 2 : Mouse Brain at 10 µg
    Lane 3 : Testis (Mouse) Whole Cell Lysate - normal tissue at 10 µg
    Lane 4 : Mouse Heart Mouse Tissue Lysate at 10 µg
    Lane 5 : Mouse skeletal muscle tissue lysate - total protein (ab29711) at 10 µg
    Lane 6 : Mouse Fetal lysate - 14 Days old Tissue Lysate at 10 µg
    Lane 7 : Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg
    Lane 8 : Mouse Embryonic Fibroblast 1 at 10 µg
    Lane 9 : NIH/3T3 whole cell lysate (ab7179) at 10 µg

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size: 38 kDa
    Observed band size: 38 kDa
    Additional bands at: 105 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
  • Western blot - Anti-Torsin A/DYT1 antibody (ab34540)
    Western blot - Anti-Torsin A/DYT1 antibody (ab34540)
    All lanes : Anti-Torsin A/DYT1 antibody (ab34540) at 1 µg/ml

    Lane 1 : NIH/3T3 whole cell lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Liver (Mouse) Tissue Lysate - normal tissue
    Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 5 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 38 kDa
    Observed band size: 38 kDa
    Additional bands at: 65 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
  • Immunocytochemistry/ Immunofluorescence - Anti-Torsin A/DYT1 antibody (ab34540)
    Immunocytochemistry/ Immunofluorescence - Anti-Torsin A/DYT1 antibody (ab34540)
    ICC/IF image of ab34540 stained SHSY5Y cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab34540, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

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