Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Torsin A/DYT1 antibody [EP2569Y] (ab76133)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2569Y] to Torsin A/DYT1
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Torsin A/DYT1 antibody [EP2569Y]
    See all Torsin A/DYT1 primary antibodies

  • Description

    Rabbit monoclonal [EP2569Y] to Torsin A/DYT1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Torsin A/DYT1 aa 300-400 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: SW480, Caco-2, A549, NIH/3T3, and C6 whole cell lysates. Rat brain and mouse lung tissue lysates; Flow Cyt: SH-SY5Y cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as Torsin A

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Torsin A/DYT1 antibody [EP2569Y] (ab76133)
    Western blot - Anti-Torsin A/DYT1 antibody [EP2569Y] (ab76133)
    All lanes : Anti-Torsin A/DYT1 antibody [EP2569Y] (ab76133) at 1/5000 dilution (purified)

    Lane 1 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : Rat brain lysate
    Lane 4 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
    Lane 5 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 6 : Mouse lung lysate
    Lane 7 : C6 (Rat glial tumor glial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 37 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?

  • Flow Cytometry - Anti-Torsin A/DYT1 antibody [EP2569Y] (ab76133)
    Flow Cytometry - Anti-Torsin A/DYT1 antibody [EP2569Y] (ab76133)

    Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Torsin A/DYT1 with purified ab76133 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Anti-Torsin A/DYT1 antibody [EP2569Y] (ab76133)
    Anti-Torsin A/DYT1 antibody [EP2569Y] (ab76133)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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