Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Sortilin/NT3 antibody [EPR23093-58] - BSA and Azide free (ab263873)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23093-58] to Sortilin/NT3 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Sortilin/NT3 antibody [EPR23093-58] - BSA and Azide free
    See all Sortilin/NT3 primary antibodies

  • Description

    Rabbit monoclonal [EPR23093-58] to Sortilin/NT3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    IHC-P
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human brain, SH-SY5Y, mouse brain, mouse lung, rat brain, rat lung, Neuro-2a, C6, RAW 264.7, PC-12 and NIH/3T3 lysates. IHC-P: Human endometrium, human ovarian carcinoma, breast carcinoma and the paired adjacent breast tissue. Mouse and rat cerebrum tissue. ICC/IF: SH-SY5Y and Neuro-2a cells. Flow Cyt: SH-SY5Y and Neuro-2a cells.

  • General notes

    ab263873 is a PBS-only buffer format of ab263864. Please refer to ab263864 for recommended dilutions, protocols, and image data.

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Images

  • Flow Cytometry - Anti-Sortilin/NT3 antibody [EPR23093-58] - BSA and Azide free (ab263873)
    Flow Cytometry - Anti-Sortilin/NT3 antibody [EPR23093-58] - BSA and Azide free (ab263873)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling Sortilin/NT3 with ab263864 at 1/600 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1\2000 was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263864).

  • Flow Cytometry - Anti-Sortilin/NT3 antibody [EPR23093-58] - BSA and Azide free (ab263873)
    Flow Cytometry - Anti-Sortilin/NT3 antibody [EPR23093-58] - BSA and Azide free (ab263873)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Sortilin/NT3 with ab263864 at 1/600 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1\2000 was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263864).

  • Immunocytochemistry/ Immunofluorescence - Anti-Sortilin/NT3 antibody [EPR23093-58] - BSA and Azide free (ab263873)
    Immunocytochemistry/ Immunofluorescence - Anti-Sortilin/NT3 antibody [EPR23093-58] - BSA and Azide free (ab263873)

    Immunofluorescent analysis of 100% methanol-fixed Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling Sortilin/NT3 with ab263864 at 1\100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1\1000 dilution (Green). Confocal image showing golgi staining in Neuro-2a cell line. 100% methanol fixation is recommended. Counterstained with ab169276 Anti-GM130 antibody-Golgi Marker (1/500 dilution) and ab150120 AlexaFluor®594 Goat anti-Mouse secondary (1/1000 dilution, red). The nuclear counterstain was DAPI (Blue).

    The negative controls are as follows:

    -ve control 1: ab263864 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/500 dilution.

    -ve control 2: ab169276 Anti-GM130 antibody- Golgi Marker at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263864).

  • Immunocytochemistry/ Immunofluorescence - Anti-Sortilin/NT3 antibody [EPR23093-58] - BSA and Azide free (ab263873)
    Immunocytochemistry/ Immunofluorescence - Anti-Sortilin/NT3 antibody [EPR23093-58] - BSA and Azide free (ab263873)

    Immunofluorescent analysis of 100% methanol-fixed SH-SY5Y (Human neuroblastoma epithelial cell) cells labelling Sortilin/NT3 with ab263864 at 1\100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1\1000 dilution (Green). Confocal image showing cytoplasmic staining in SH-SY5Y cell line. 100% methanol fixation is recommended. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1\1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263864).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [EPR23093-58] - BSA and Azide free (ab263873)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [EPR23093-58] - BSA and Azide free (ab263873)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Sortilin/NT3 with ab263864 at 1/5000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat cerebrum is observed. The section was incubated with ab263864 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263864).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [EPR23093-58] - BSA and Azide free (ab263873)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [EPR23093-58] - BSA and Azide free (ab263873)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Sortilin/NT3 with ab263864 at 1/5000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse cerebrum iso bserved. The section was incubated with ab263864 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263864).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [EPR23093-58] - BSA and Azide free (ab263873)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [EPR23093-58] - BSA and Azide free (ab263873)

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma and the paired adjacent breast tissue tissue labeling Sortilin/NT3 with ab263864 at 1/5000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human breast carcinoma (panel A) and weak staining on the paired adjacent breast tissue (panel B) (PMID: 25871389) is observed. The section was incubated with ab263864 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263864).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [EPR23093-58] - BSA and Azide free (ab263873)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [EPR23093-58] - BSA and Azide free (ab263873)

    Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue labeling Sortilin/NT3 with ab263864 at 1/5000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human ovarian carcinoma (PMID: 23407681) is observed. The section was incubated with ab263864 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263864).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [EPR23093-58] - BSA and Azide free (ab263873)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [EPR23093-58] - BSA and Azide free (ab263873)

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling Sortilin/NT3 with ab263864 at 1/5000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human endometrium (PMID: 25662808) is observed. The section was incubated with ab263864 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263864).

  • Anti-Sortilin/NT3 antibody [EPR23093-58] - BSA and Azide free (ab263873)
    Anti-Sortilin/NT3 antibody [EPR23093-58] - BSA and Azide free (ab263873)

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