Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GAD65 antibody [EPR22952-70] - BSA and Azide free (ab270037)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22952-70] to GAD65 - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB, IHC-Fr, IP, Flow Cyt
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-GAD65 antibody [EPR22952-70] - BSA and Azide free
    See all GAD65 primary antibodies

  • Description

    Rabbit monoclonal [EPR22952-70] to GAD65 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC
    Mouse
    IHC-Fr
    Mouse
    IHC-P
    Mouse
    IP
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Rat pancreas and Mouse cerebrum tissues. IHC-Fr: Rat pancreas and Mouse cerebrum tissues. Flow Cyt: Beta-TC-6IP: Mouse brain lysate.

  • General notes

    ab270037 is the carrier-free version of ab239372. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-GAD65 antibody [EPR22952-70] - BSA and Azide free (ab270037)
    Immunohistochemistry (Frozen sections) - Anti-GAD65 antibody [EPR22952-70] - BSA and Azide free (ab270037)

    Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Rat pancreas tissue labeling GAD65 with ab239372 at 1/100 dilution (Green) followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab239372).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 antibody [EPR22952-70] - BSA and Azide free (ab270037)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 antibody [EPR22952-70] - BSA and Azide free (ab270037)

    Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue labeling GAD65 with ab239372 at 1/2000 (0.323 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on islet of rat pancreas. The section was incubated with ab239372 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab239372).

  • Immunohistochemistry (Frozen sections) - Anti-GAD65 antibody [EPR22952-70] - BSA and Azide free (ab270037)
    Immunohistochemistry (Frozen sections) - Anti-GAD65 antibody [EPR22952-70] - BSA and Azide free (ab270037)

    Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Mouse cerebellum tissue labeling GAD65 with ab239372 at 1/100 dilution (Green) followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Positive staining on mouse cerebellum is observed.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab239372).

  • Immunocytochemistry - Anti-GAD65 antibody [EPR22952-70] - BSA and Azide free (ab270037)
    Immunocytochemistry - Anti-GAD65 antibody [EPR22952-70] - BSA and Azide free (ab270037)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling GAD65 with ab239372 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neurons.is observed. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue). Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab239372).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 antibody [EPR22952-70] - BSA and Azide free (ab270037)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 antibody [EPR22952-70] - BSA and Azide free (ab270037)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling GAD65 with ab239372 at 1/2000 (0.323 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebrum is observed. The section was incubated with ab239372 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab239372).

  • Flow Cytometry - Anti-GAD65 antibody [EPR22952-70] - BSA and Azide free (ab270037)
    Flow Cytometry - Anti-GAD65 antibody [EPR22952-70] - BSA and Azide free (ab270037)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Beta-TC-6 (Mouse pancreas insulinoma beta cell) cells labelling GAD65 with ab239372 at 1/60 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab239372).

  • Immunoprecipitation - Anti-GAD65 antibody [EPR22952-70] - BSA and Azide free (ab270037)
    Immunoprecipitation - Anti-GAD65 antibody [EPR22952-70] - BSA and Azide free (ab270037)

    GAD65 was immunoprecipitated from 0.35 mg Mouse brain lysate with ab239372 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab239372 at 1/1000 dilution (0.45 μg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse brain lysate 10 μg

    Lane 2: ab239372 IP in Mouse brain lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab239372 in Mouse brain lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds

    Bands above 100kDa are multimers of GAD65 (PMID: 10601283).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab239372).

  • Anti-GAD65 antibody [EPR22952-70] - BSA and Azide free (ab270037)
    Anti-GAD65 antibody [EPR22952-70] - BSA and Azide free (ab270037)

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