Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18278-222] to STAT6 - BSA and Azide free
  • Suitable for: ICC, IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free
    See all STAT6 primary antibodies

  • Description

    Rabbit monoclonal [EPR18278-222] to STAT6 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251555 is the carrier-free version of ab217998. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251555 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
    Western blot - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
    All lanes : Anti-STAT6 antibody [EPR18278-222] (ab217998) at 1/1000 dilution

    Lane 1 : Human thymus lysate
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : P388D1 (Mouse lymphoma N106 O91monocyte; macrophage cell line) whole cell lysate
    Lane 4 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate
    Lane 5 : THP-1 (Human monocytic leukemia cell line) whole cell lysate
    Lane 6 : Daudi (Human Burkitt's lymphoma cell line) whole cell lysate
    Lane 7 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 94 kDa
    Observed band size: 110,75,81 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab217998, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-3: 8 seconds; Lanes 4-5: 2 seconds; Lanes 6-7: 1 second.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 21411736). This antibody recognizes three isoforms of STAT6, isoform 1 (110kDa), isoform 2 (75kDa), isoform 3 (81kDa), which is consistent with the Uniprot annotation.

  • Immunocytochemistry - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
    Immunocytochemistry - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
    This data was developed using ab217998, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 100% methanol-fixed Raji (Human Burkitt's lymphoma cell line) cells labeling STAT6 with ab217998 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and nuclear staining on Raji cell line [PMID: 11781246] [PMID: 23575898]. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
  • Immunocytochemistry - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
    Immunocytochemistry - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
    This data was developed using ab217998, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 100% methanol-fixed Daudi (Human Burkitt's lymphoma cell line) cells labeling STAT6 with ab217998 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and nuclear staining on Daudi cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
  • Western blot - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
    Western blot - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
    All lanes : Anti-STAT6 antibody [EPR18278-222] (ab217998) at 1/2000 dilution

    Lane 1 : Mouse spleen tissue lysate
    Lane 2 : Rat spleen tissue lysate
    Lane 3 : A20 (Mouse reticulum sarcoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 94 kDa
    Observed band size: 110,75,81 kDa why is the actual band size different from the predicted?



    This data was developed using ab217998, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 10 seconds; Lane 2: 3 seconds; Lane 3: 5 seconds.

  • Immunoprecipitation - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
    Immunoprecipitation - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)

    This data was developed using ab217998, the same antibody clone in a different buffer formulation.

    STAT6 was immunoprecipitated from 0.35 mg of Raji (Human Burkitt's lymphoma cell line) whole cell lysate with ab217998 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab217998 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Raji whole cell lysate. 10 µg (Input).

    Lane 2: ab217998 IP in Raji whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab217998 in Raji whole cell lysate.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 1 second.

    This antibody recognizes three isoforms of STAT6, isoform 1 (110kDa), isoform 2 (75kDa), isoform 3 (81kDa), respectively which is consistent with the Uniprot annotation.

  • Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
    Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)

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