Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-STAT1 antibody [EPRR21057-168] - BSA and Azide free (ab234902)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPRR21057-168] to STAT1 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-STAT1 antibody [EPRR21057-168] - BSA and Azide free
    See all STAT1 primary antibodies

  • Description

    Rabbit monoclonal [EPRR21057-168] to STAT1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil tissue.

  • General notes

    Ab234902 is the carrier-free version of ab210524. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab234902 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Flow Cytometry - Anti-STAT1 antibody [EPRR21057-168] - BSA and Azide free (ab234902)
    Flow Cytometry - Anti-STAT1 antibody [EPRR21057-168] - BSA and Azide free (ab234902)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized MCF7 (Human breast adenocarcinoma cell line) cell line labeling STAT1 with ab210524 at 1/500 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210524).

  • Immunoprecipitation - Anti-STAT1 antibody [EPRR21057-168] - BSA and Azide free (ab234902)
    Immunoprecipitation - Anti-STAT1 antibody [EPRR21057-168] - BSA and Azide free (ab234902)

    STAT1 was immunoprecipitated from 0.35 mg MCF7 (Human breast adenocarcinoma cell line) whole cell lysate with ab210524 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab210524 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
    Lane 1: MCF7 whole cell lysate 10 µg (Input).
    Lane 2: ab210524 IP in MCF7 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab210524 in MCF7 whole cell lysate (-).

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 15 seconds.

    The doublet observed in some lanes likely represent the α and β isoforms of STAT1 (PMID: 8647800).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210524).

  • Flow Cytometry - Anti-STAT1 antibody [EPRR21057-168] - BSA and Azide free (ab234902)
    Flow Cytometry - Anti-STAT1 antibody [EPRR21057-168] - BSA and Azide free (ab234902)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cell line labeling STAT1 with ab210524 at 1/500 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210524).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 antibody [EPRR21057-168] - BSA and Azide free (ab234902)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 antibody [EPRR21057-168] - BSA and Azide free (ab234902)

    Immunohistochemical analysis of paraffin-embedded paired human endometrial cancer and non-tumor endometrium tissue labeling STAT1 with ab210524 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Much higher staining intensity of endometrial cancer (Panel A) than its paired non-tumor endometrium (Panel B) (PMID: 25267067) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210524).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 antibody [EPRR21057-168] - BSA and Azide free (ab234902)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 antibody [EPRR21057-168] - BSA and Azide free (ab234902)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling STAT1 with ab210524 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on glomerulus and some renal tubules of human kidney (PMID: 26678048) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210524).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 antibody [EPRR21057-168] - BSA and Azide free (ab234902)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 antibody [EPRR21057-168] - BSA and Azide free (ab234902)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling STAT1 with ab210524 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining mainly on interfollicular cells of human tonsil (PMID: 25336386; PMID: 25921060) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210524).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-STAT1 antibody [EPRR21057-168] - BSA and Azide free (ab234902)
    Anti-STAT1 antibody [EPRR21057-168] - BSA and Azide free (ab234902)

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