Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-STAT6 antibody [EPR1912] - BSA and Azide free (ab248745)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1912] to STAT6 - BSA and Azide free
  • Suitable for: ICC, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-STAT6 antibody [EPR1912] - BSA and Azide free
    See all STAT6 primary antibodies

  • Description

    Rabbit monoclonal [EPR1912] to STAT6 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248745 is the carrier-free version of ab134940 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248745 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-STAT6 antibody [EPR1912] - BSA and Azide free (ab248745)
    Western blot - Anti-STAT6 antibody [EPR1912] - BSA and Azide free (ab248745)

    This data was developed using ab134940, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)

    Lane 2: STAT6 knockout HAP1 whole cell lysate (20 µg)

    Lane 3: HeLa whole cell lysate (20 µg)

    Lane 4: Raji whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab134940 observed at 94 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab134940 was shown to recognize STAT6 in wild-type HAP1 cells as signal was lost at the expected MW in STAT6 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and STAT6 knockout samples were subjected to SDS-PAGE. ab134940 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry - Anti-STAT6 antibody [EPR1912] - BSA and Azide free (ab248745)
    Immunocytochemistry - Anti-STAT6 antibody [EPR1912] - BSA and Azide free (ab248745)
    This data was developed using ab134940, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of Daudi cells, labelling STAT6 using ab134940 at a 1/100 dilution (green). Cell nuclei are shown in blue.
  • Western blot - Anti-STAT6 antibody [EPR1912] - BSA and Azide free (ab248745)
    Western blot - Anti-STAT6 antibody [EPR1912] - BSA and Azide free (ab248745)
    All lanes : Anti-STAT6 antibody [EPR1912] (ab134940) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Human lymph node lysate
    Lane 3 : Daudi cell lysate
    Lane 4 : Raji cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 94 kDa



    This data was developed using ab134940, the same antibody clone in a different buffer formulation.

     

  • Anti-STAT6 antibody [EPR1912] - BSA and Azide free (ab248745)
    Anti-STAT6 antibody [EPR1912] - BSA and Azide free (ab248745)

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