Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3133] to CD90 / Thy1 - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free
    See all CD90 / Thy1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3133] to CD90 / Thy1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human fetal brain and Human bone marrow lysates. Human glioma and kidney tissues

  • General notes

    ab226123 is the carrier-free version of ab133350. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab226123 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)

    Immunohistochemical analysis of paraffin-embedded Human normal kidney tissue labelling CD90 / Thy1 with ab133350 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133350).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)
    Immunocytochemistry/ Immunofluorescence - Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)

    ab133350 staining CD90 / Thy1  in the SH-SY5Y human neuroblastoma epithelial cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/100). ab150077 an Alexa Fluor® 488-conjugated Goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI was used as a nuclear counter stain and Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counter stain microtubules.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133350).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)

    Immunohistochemical analysis of paraffin-embedded Human glioma tissue labelling CD90 / Thy1 with ab133350 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133350).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)

    Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using ab133350 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133350).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)

    Immunohistochemical analysis of paraffin embedded normal Human tonsil vessels tissue using ab133350 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133350).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)

    Immunohistochemical analysis of paraffin embedded Human Lung adenocarcinoma tissue using ab133350 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133350).

  • Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)
    Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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