Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD90 / Thy1 antibody [EPR3133] - Low endotoxin, Azide free (ab216449)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3133] to CD90 / Thy1 - Low endotoxin, Azide free
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-CD90 / Thy1 antibody [EPR3133] - Low endotoxin, Azide free
    See all CD90 / Thy1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3133] to CD90 / Thy1 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC: Human tonsil tissue; WB: Human glioma and brain tissue lysate; ICC: Jurkat cells.

  • General notes

    ab216449 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-CD90 / Thy1 antibody [EPR3133] - Low endotoxin, Azide free (ab216449)
    Western blot - Anti-CD90 / Thy1 antibody [EPR3133] - Low endotoxin, Azide free (ab216449)
    All lanes : Anti-CD90 / Thy1 antibody [EPR3133] (ab133350) at 1/1000 dilution (Purified)

    Lane 1 : Human glioma lysate
    Lane 2 : Human brain lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 17 kDa
    Observed band size: 25-35 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab133350, the same antibody clone in a different buffer formulation.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 24116172 and PMID: 30177788).

    Blocking Buffer and concentration: 5% NFDM/TBST

  • Immunocytochemistry - Anti-CD90 / Thy1 antibody [EPR3133] - Low endotoxin, Azide free (ab216449)
    Immunocytochemistry - Anti-CD90 / Thy1 antibody [EPR3133] - Low endotoxin, Azide free (ab216449)

    This data was developed using ab133350, the same antibody clone in a different buffer formulation.
    Immunocytochemistry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling CD90 / Thy1 with Purified ab216449 at 1:50 dilution (2.44 ?g/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] - Low endotoxin, Azide free (ab216449)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] - Low endotoxin, Azide free (ab216449)

    This data was developed using ab133350, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling CD90 / Thy1 with Purified ab216449 at 1:4000 dilution (0.03 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Anti-CD90 / Thy1 antibody [EPR3133] - Low endotoxin, Azide free (ab216449)
    Anti-CD90 / Thy1 antibody [EPR3133] - Low endotoxin, Azide free (ab216449)

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