Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Immunology Adaptive Immunity T Cells CD

Anti-CD40 antibody (ab13545)

Price and availability

348 441 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-CD40 antibody (ab13545)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to CD40
  • Suitable for: WB
  • Reacts with: Human, Pig
  • Isotype: IgG

You may also be interested in

Product image
PE Anti-CD200R antibody [OX102] (ab34135)
Product image
Mouse CD27 Antibody Pair - BSA and Azide free (ab244092)
Recombinant Mouse CD83 protein (Fc Chimera) (ab214598)
Product image
Anti-CD38 antibody [90] (ab61400)

Overview

  • Product name

    Anti-CD40 antibody
    See all CD40 primary antibodies
  • Description

    Rabbit polyclonal to CD40
  • Host species

    Rabbit
  • Specificity

    The antibody specificity is confirmed by specific peptide inhibition in immunoblotting studies.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human, Pig
    Predicted to work with: Chicken, Dog
  • Immunogen

    Synthetic peptide:

    CQPVTQEDGKESRISVQERQ

    conjugated to KLH, corresponding to amino acids 258-277 of Human CD40.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    Storage below -20°C is not recommended. For maximum product recovery centrifuge the product vial before removing cap. Shipped on gel packs.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Peptide affinity purified.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Innate and adaptive immunity
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Dendritic Cell Lineage

Images

  • Western blot - Anti-CD40 antibody (ab13545)
    Western blot - Anti-CD40 antibody (ab13545)
    All lanes : Anti-CD40 antibody (ab13545) at 1/200 dilution

    Lane 1 : ESK4 cell lysate
    Lane 2 : Molt 4 cell lysate

    Predicted band size: 34 kDa

  • Western blot - Anti-CD40 antibody (ab13545)
    Western blot - Anti-CD40 antibody (ab13545) This image is courtesy of an anonymous Abreview
    All lanes : Anti-CD40 antibody (ab13545) at 1/5000 dilution

    Lane 1 : Human B chronic lymphocytic leukaemia cells, nuclear fraction
    Lane 2 : Human B chronic lymphocytic leukaemia cells, cytoskeletal fraction
    Lane 3 : Human B chronic lymphocytic leukaemia cells, membrane fraction
    Lane 4 : Human B chronic lymphocytic leukaemia cells, cytosolic fraction

    Lysates/proteins at 18 µg per lane.

    Secondary
    All lanes : HRP conjugated goat anti-rabbit

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 34 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    The primary antibody was incubated with the membrane for 16 hours at 4°C.

    See Abreview

  • Western blot - Anti-CD40 antibody (ab13545)
    Western blot - Anti-CD40 antibody (ab13545)
    Anti-CD40 antibody (ab13545) at 1/1000 dilution + Recombinant human CD40 protein (Fc Chimera Active) (ab83917) at 1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 34 kDa


    Exposure time: 8 minutes
  • Western blot - Anti-CD40 antibody (ab13545)
    Western blot - Anti-CD40 antibody (ab13545)
    All lanes : Anti-CD40 antibody (ab13545) at 1/1000 dilution

    Lanes 1-2 : Control human HCAEC cells - whole cell lysate
    Lanes 3-4 : Treated human HCAEC cells - whole cell lysate

    Secondary
    All lanes : HRP conjugated Goat Anti-Rabbit IgG

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 34 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?


    Exposure time: 20 seconds


    This image is courtesy of an Abreview submitted on 10 August 2005.

    See Abreview

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-CD40 antibody (ab13545)

  •  
  • Product image

    Anti-CD40 antibody [EPR20540] - Low endotoxin, Azide free (ab223546)

    Applications: IHC-P, Mass Cytometry, WB

  •  
  • Product image

    Anti-CD40 antibody [EPR18005-35] (ab188181)

    Applications: Flow Cyt, ICC/IF, WB

  •  
  • Product image

    Anti-CD40 antibody [EPR18005-4] (ab212058)

    Applications: Flow Cyt, ICC/IF, WB

  •  
  • Product image

    Anti-CD40 antibody [EPR20735] - BSA and Azide free (ab228818)

    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

  •  
  • Product image

    Anti-CD40 antibody [EPR18005-4] - BSA and Azide free (ab227905)

    Applications: Flow Cyt, ICC/IF, WB

  •  
  • Product image

    Anti-CD40 antibody [EPR20735] (ab224639)

    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

  •  
  • Product image

    Anti-CD40 antibody [EPR20540] (ab213205)

    Applications: IHC-P, WB

Clear all

Recently viewed products

  •  
  • Product image

    Anti-Tensin 3 antibody (ab178554)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.