Anti-CD40 antibody (ab13545)
Key features and details
- Rabbit polyclonal to CD40
- Suitable for: WB
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-CD40 antibody
See all CD40 primary antibodies -
Description
Rabbit polyclonal to CD40 -
Host species
Rabbit -
Specificity
The antibody specificity is confirmed by specific peptide inhibition in immunoblotting studies. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Chicken, Dog -
Immunogen
Synthetic peptide:
CQPVTQEDGKESRISVQERQ
conjugated to KLH, corresponding to amino acids 258-277 of Human CD40. -
General notes
Storage below -20°C is not recommended. For maximum product recovery centrifuge the product vial before removing cap. Shipped on gel packs.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Peptide affinity purified. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-CD40 antibody (ab13545) at 1/200 dilution
Lane 1 : ESK4 cell lysate
Lane 2 : Molt 4 cell lysate
Predicted band size: 34 kDa
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All lanes : Anti-CD40 antibody (ab13545) at 1/5000 dilution
Lane 1 : Human B chronic lymphocytic leukaemia cells, nuclear fraction
Lane 2 : Human B chronic lymphocytic leukaemia cells, cytoskeletal fraction
Lane 3 : Human B chronic lymphocytic leukaemia cells, membrane fraction
Lane 4 : Human B chronic lymphocytic leukaemia cells, cytosolic fraction
Lysates/proteins at 18 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThe primary antibody was incubated with the membrane for 16 hours at 4°C.
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Anti-CD40 antibody (ab13545) at 1/1000 dilution +
Recombinant human CD40 protein (Fc Chimera Active) (ab83917) at 1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Exposure time: 8 minutes -
All lanes : Anti-CD40 antibody (ab13545) at 1/1000 dilution
Lanes 1-2 : Control human HCAEC cells - whole cell lysate
Lanes 3-4 : Treated human HCAEC cells - whole cell lysate
Secondary
All lanes : HRP conjugated Goat Anti-Rabbit IgG
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 20 seconds
This image is courtesy of an Abreview submitted on 10 August 2005.