Recombinant human CD80 protein (ab180050)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 95% PBS, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
Images
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Functional Studies - Recombinant human CD80 protein (ab180050)Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) on CM5 Chip can bind Human B7-1, His Tag with an affinity constant of 5.29 µM as determined in a SPR assay (Biacore T200).
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SDS-PAGE - Recombinant human CD80 protein (ab180050)Human B7-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%. -
Functional Studies - Recombinant human CD80 protein (ab180050)Immobilized Human CTLA-4, Fc Tag at 10 µg/mL (100 µL/well) can bind Human B7-1, His Tag with a linear range of 0.156-1.25 µg/mL. -
SDS-PAGE - Recombinant human CD80 protein (ab180050)SDS-PAGE analysis of ab180050 in reducing conditions. Gel stained overnight with Coomassie Blue. Protein migrates as 45 - 75 kDa due to different glycosylation.
