Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant human CD80 protein (Fc Chimera Active) (ab214978)

Key features and details

  • Expression system: CHO cells
  • Purity: >= 98% SDS-PAGE
  • Endotoxin level:
  • Active: Yes
  • Suitable for: Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant human CD80 protein (Fc Chimera Active)
    See all CD80 proteins and peptides

  • Biological activity

    Measured by its ability to induce IL-2 secretion by Jurkat Human acute T cell leukemia cells.

  • Purity

    >= 98 % SDS-PAGE.

  • Endotoxin level

  • Expression system

    CHO cells

  • Accession

    P33681

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      VIHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIW PEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEV TLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNA INTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQTFNWNTT KQEHFPDN

    • Predicted molecular weight

      24 kDa

    • Amino acids

      35 to 242

    • Additional sequence information

      Extracellular domain, fused to the N-terminus of the Fc region of mouse IgG2a (NP_005182.1).

    • Associated products

      Specifications

      Our Abpromise guarantee covers the use of ab214978 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      • Applications

        Functional Studies

        SDS-PAGE

      • Form

        Lyophilized
      • Concentration information loading...

      Preparation and Storage

      • Stability and Storage

        Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.

        Constituent: 99% PBS

        Lyophilized from 0.2 µm filtered solution.

        This product is an active protein and may elicit a biological response in vivo, handle with caution.

      • Reconstitution
        Reconstitute with 100 µL sterile water. Add 1X PBS to the desired protein concentration. Working aliquots are stable for up to 3 months when stored at -20°C.

      General Info

      • Alternative names

        • Activation B7-1 antigen
        • B lymphocyte activation antigen B7
        • B7
        • B7-1
        • B7-1 antigen
        • B7.1
        • BB1
        • CD28 antigen ligand 1
        • CD28LG
        • CD28LG1
        • CD80
        • CD80 antigen
        • CD80 antigen (CD28 antigen ligand 1, B7-1 antigen)
        • CD80 molecule
        • CD80_HUMAN
        • Costimulatory factor CD80
        • costimulatory molecule variant IgV-CD80
        • CTLA-4 counter-receptor B7.1
        • LAB7
        • T-lymphocyte activation antigen CD80
        see all

      • Function

        Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

      • Tissue specificity

        Expressed on activated B-cells, macrophages and dendritic cells.

      • Sequence similarities

        Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
        Contains 1 Ig-like V-type (immunoglobulin-like) domain.

      • Cellular localization

        Membrane.
      • Information by UniProt

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab214978? Please let us know so that we can cite the reference in this datasheet.

    ab214978 has not yet been referenced specifically in any publications.

    Preparation and Storage

    • Alternative names

      • Activation B7-1 antigen
      • B lymphocyte activation antigen B7
      • B7
      • B7-1
      • B7-1 antigen
      • B7.1
      • BB1
      • CD28 antigen ligand 1
      • CD28LG
      • CD28LG1
      • CD80
      • CD80 antigen
      • CD80 antigen (CD28 antigen ligand 1, B7-1 antigen)
      • CD80 molecule
      • CD80_HUMAN
      • Costimulatory factor CD80
      • costimulatory molecule variant IgV-CD80
      • CTLA-4 counter-receptor B7.1
      • LAB7
      • T-lymphocyte activation antigen CD80
      see all

    • Function

      Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

    • Tissue specificity

      Expressed on activated B-cells, macrophages and dendritic cells.

    • Sequence similarities

      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    • Cellular localization

      Membrane.
    • Information by UniProt

    Images

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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